Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt
Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt (CAS 856661-92-2) is a specialised chemical compound with the empirical formula C26H40K3N5O9S. It functions as a hetero-bifunctional linker, crucial in biochemical research for connecting proteins with specific tags to detection or purification molecules. Supplied by TSS, this reagent aids in various protein interaction studies and detection assays.
- IUPAC
- Biotin-X-NTA
- Synonyms
- C26H40K3N5O9S
Protein Ligation and Labelling
This compound acts as a hetero-bifunctional linker, enabling the covalent attachment between proteins, particularly those with a Histidine-tag (His-tag), and avidin or streptavidin conjugates. This is essential for downstream detection, purification, or immobilisation of tagged proteins.
Biochemical Assays and Research
Its utility extends to various biochemical research applications, including proximity ligation assays, proximity extension assays, and other molecular biology techniques where specific protein-protein interactions or protein-tag binding needs to be facilitated and detected.
Histidine-Tagged Protein Applications
Specifically designed for use with Histidine-tagged proteins, this linker facilitates the robust coupling of these proteins to molecules conjugated with biotin or streptavidin, enhancing signal amplification or immobilisation strategies in experiments.
| Empirical formula | C26H40K3N5O9S |
|---|---|
| Assay | ≥98.0% (TLC) |
| Application | Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine (Biotin-X-NTA) is used as a hetero-bifunctional linker between histone(His)-tagged proteins and (strept)avidin conjugates. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt used for?
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This compound is used as a hetero-bifunctional linker, connecting His-tagged proteins to (strept)avidin conjugates for detection and research purposes.
What are the CAS number and formula for this chemical?
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The CAS number is 856661-92-2 and the empirical formula is C26H40K3N5O9S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥98.0% (TLC). We do not represent it as USP, BP, or EP grade.
What are the safety and handling recommendations?
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Handle with care, using appropriate personal protective equipment such as eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound is classified with WGK Germany 3, indicating a high hazard to water.
How is this product packed, shipped, and exported?
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Tech Serve Solutions specialises in global supply and export of fine chemicals and reagents, ensuring appropriate packaging and adherence to international shipping regulations for hazardous materials.
How can I request a sample or quote for this product?
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Please contact our customer service team via the website or phone to request a sample or a detailed quotation for Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt.
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