N-Acetyl-L-leucine-p-nitroanilide
N-Acetyl-L-leucine-p-nitroanilide (CAS 19746-40-8; C14H19N3O4; molar mass 293.32 g/mol) is a chromogenic enzyme substrate supplied by Tech Serve Solutions for protease and aminopeptidase activity assays. On enzymatic cleavage of the anilide bond it releases yellow p-nitroaniline, measured spectrophotometrically near 405 nm to give a sensitive, continuous read-out of leucine-aminopeptidase-type activity. It is offered as a defined research-grade reagent for biochemical and analytical laboratories.
- Synonyms
- N-Acetyl-L-leucine-p-nitroanilideN-Acetyl-L-leucine 4-nitroanilideAc-Leu-pNAAcetyl-L-leucine p-nitroanilide

Chromogenic protease substrate
Used as a colorimetric substrate to measure aminopeptidase and protease activity; cleavage liberates p-nitroaniline, read at about 405 nm as a direct index of enzyme activity.
Enzyme kinetics and inhibitor screening
Supports kinetic characterisation and inhibitor screening of leucine-aminopeptidase-type enzymes in research and assay development.
Biochemical research reagent
A defined modified-amino-acid reagent for protein chemistry, enzymology and analytical method development.
| Molecular weight | 293.32 |
|---|---|
| Empirical formula | C14H19N3O4 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Acetyl-L-leucine-p-nitroanilide used for?
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It is a chromogenic substrate for assaying aminopeptidase and protease activity. Enzymatic cleavage releases p-nitroaniline, a yellow chromophore measured spectrophotometrically (around 405 nm), enabling sensitive enzyme-activity and kinetic studies.
What is the CAS number and molecular formula?
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The CAS number is 19746-40-8 and the molecular formula is C14H19N3O4, with a molar mass of 293.32 g/mol.
What grade and purity does Tech Serve Solutions supply?
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It is supplied as a defined research/reagent-grade biochemical, with a certificate of analysis available on request. We do not represent it as a USP, BP or EP pharmacopoeia grade.
How should it be stored and handled?
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Store sealed at -20 degrees C, protected from moisture, and handle with standard laboratory protection (gloves, eye protection, dust precautions) in line with the Safety Data Sheet.
How is it packed, shipped and exported?
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It ships as a packaged solid with full export documentation. As a specialist supplier and exporter, Tech Serve Solutions delivers worldwide, including to the US, Europe and Asia.
How do I request a sample or quote?
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Request a sample for evaluation or a bulk quotation through our contact form, stating your required quantity and destination; we confirm specification, lead time and export terms.
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