MTT Formazan
MTT Formazan (CAS 57360-69-7, C18H17N5S) is a chromogenic substrate widely employed in biochemical assays. It is metabolised by dehydrogenases in living cells to produce a soluble, coloured formazan product, enabling the assessment of cellular metabolic activity and viability. This compound is integral to numerous research applications, particularly in drug discovery and toxicology screening, offering a quantitative measure of cellular health.
- IUPAC
- 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan,Thiazolyl blue formazan
- Synonyms
- Thiazolyl blue formazan1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan
Cell Viability Assays
MTT Formazan is a key reagent for colorimetric assays that measure mitochondrial activity as an indicator of cell viability. The formazan product's intensity is directly proportional to the number of viable cells present in a culture.
Drug Discovery and Screening
Utilised in high-throughput screening to evaluate the cytotoxic or cytostatic effects of potential drug candidates. Its sensitivity allows for the detection of subtle changes in cellular metabolism induced by compounds.
Toxicology Studies
Applied in toxicity testing to determine the adverse effects of chemicals on cellular function. It provides a quantitative measure of cell damage or inhibition of metabolic processes.
Enzyme Activity Measurement
Serves as a substrate for certain dehydrogenase enzymes, enabling the quantification of their activity in biological samples. The resulting coloured product allows for spectrophotometric analysis.
| Molecular weight | 335.43 |
|---|---|
| Empirical formula | C18H17N5S |
| Assay | ≥97% (TLC) |
| Solubility | acetone: soluble1 mg/mL |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is MTT Formazan used for?
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MTT Formazan is primarily used as a chromogenic substrate in assays to assess cellular metabolic activity and viability, commonly in drug discovery, toxicology studies, and enzyme activity measurements.
What is the CAS number and formula for MTT Formazan?
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The CAS number for MTT Formazan is 57360-69-7, and its empirical formula is C18H17N5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies MTT Formazan with an assay of ≥97% (TLC). This product is intended for research purposes and is not represented as USP, BP, EP, or other pharmacopoeial grades.
What are the safety considerations for handling MTT Formazan?
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When handling MTT Formazan, appropriate personal protective equipment is essential. This includes eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter to protect against potential exposure. Ensure adequate ventilation.
Is MTT Formazan a controlled substance?
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The provided data does not indicate that MTT Formazan (CAS 57360-69-7) is a controlled substance.
How is MTT Formazan packed and shipped?
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Tech Serve Solutions packs and ships fine chemicals, pharmaceutical intermediates, and laboratory reagents globally, adhering to relevant regulations for safe transport.
How can I request a quote or sample of MTT Formazan?
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To request a quote or sample of MTT Formazan, please contact our sales team through the website or by phone, providing your specific requirements.
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