Methylene Blue hydrate
Methylene Blue hydrate (CAS: 122965-43-9), with the empirical formula C16H18ClN3S · xH2O, is a versatile chemical compound. It is known for its strong dyeing properties and its utility as a redox indicator. This hydrated form finds applications in various scientific and industrial fields due to its unique chemical characteristics, including its use in biological staining and as a reagent in specific analytical procedures.

Biological Staining
Methylene Blue hydrate is widely used as a vital stain in histology and microscopy to visualise cellular structures. Its ability to selectively stain certain tissues and microorganisms makes it invaluable in diagnostic procedures and research.
Redox Indicator
The compound functions as an effective redox indicator, changing colour based on the oxidation state of the solution. This property is useful in titrations and other analytical chemistry applications where monitoring redox potential is crucial.
Blood Stain Suitability
This particular formulation of Methylene Blue hydrate is suitable for use as a blood stain. It aids in the examination and identification of blood cells and potential abnormalities.
Research Reagent
As a biochemical reagent, Methylene Blue hydrate is employed in various research settings, including studies related to cell biology and nitric oxide metabolism, owing to its bioactive properties.
| Molecular weight | 319.85 (anhydrous basis) |
|---|---|
| Empirical formula | C16H18ClN3S · xH2O |
| Assay | (IR spectrum consistent with structure) |
| Suitability | suitable for blood stain |

Hazard statements
- H302Harmful if swallowed
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 661 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methylene Blue hydrate used for?
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Methylene Blue hydrate is used for biological staining, as a redox indicator, in blood stain applications, and as a research reagent in fields such as cell biology and nitric oxide metabolism.
What are the CAS number and formula for Methylene Blue hydrate?
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The CAS number for Methylene Blue hydrate is 122965-43-9, and its empirical formula is C16H18ClN3S · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methylene Blue hydrate with an assay consistent with IR spectrum identification. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for Methylene Blue hydrate?
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Methylene Blue hydrate is classified with a 'Warning' signal word and carries the hazard statement H302 (Harmful if swallowed). Recommended personal protective equipment includes a dust mask type N95 (US), eyeshields, and gloves.
How is Methylene Blue hydrate packed, shipped, and exported?
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Tech Serve Solutions reliably packs, ships, and exports Methylene Blue hydrate globally, adhering to all necessary regulations for chemical transport.
How can I request a sample or quote for Methylene Blue hydrate?
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To request a sample or a quote for Methylene Blue hydrate, please contact our sales team through the website or direct inquiry channels.
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