Methyl Orange
Methyl Orange (CAS: 547-58-0, Formula: C14H14N3NaO3S, MW: 327.33) is a certified biological stain and pH indicator. This azo dye exhibits a distinct colour change from pink to yellow as the pH shifts from acidic to alkaline, with a transition range of 3.0-4.4. Its reliable performance makes it a valuable reagent in chemical analysis and biological staining procedures. Tech Serve Solutions offers this high-quality chemical for diverse laboratory applications.
- IUPAC
- 4-[4-(Dimethylamino)phenylazo]benzenesulfonic acid sodium salt,Acid Orange 52,Helianthin,Orange III
- Synonyms
- 4-[4-(Dimethylamino)phenylazo]benzenesulfonic acid sodium saltAcid Orange 52HelianthinOrange III

pH Indicator
Methyl Orange serves as a visual indicator in acid-base titrations. It changes colour within a specific pH range (3.0-4.4), allowing for accurate determination of the equivalence point.
Biological Staining
Certified by the Biological Stain Commission, Methyl Orange is used in histology and hematology for differential staining of cellular components. It helps in visualizing tissue structures and cell morphology.
Chemical Analysis
Its sharp colour transition is utilised in various analytical chemistry techniques requiring precise pH monitoring or endpoint detection.
| Molecular weight | 327.33 |
|---|---|
| Empirical formula | C14H14N3NaO3S |
| Grade | certified by the Biological Stain Commission |
| Composition | Dye content, ≥85% |

Hazard statements
- H301Toxic if swallowed
Precautionary statements
- P301IF SWALLOWED
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 3143PSN1 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 25 |
| Safety statements (S) | 45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 13025 |
|---|---|
| Absorption | λmax 505 nm |
| Ph Range | 3.0 - 4.4, pink to yellow |
| Synonym | 3.2 - 4.4, red to yellow |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methyl Orange used for?
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Methyl Orange is primarily used as a pH indicator in acid-base titrations and for biological staining in histology and hematology.
What is the CAS number and formula for Methyl Orange?
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The CAS number for Methyl Orange is 547-58-0, and its molecular formula is C14H14N3NaO3S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methyl Orange that is certified by the Biological Stain Commission, with a dye content of ≥85%. We do not represent this product as USP, BP, or EP grade.
How should Methyl Orange be handled safely?
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Methyl Orange is a toxic substance (GHS06, Signal Word: Danger, H301). Handle with appropriate personal protective equipment, including eyeshields, faceshields, gloves, and a P2 respirator cartridge. Ensure adequate ventilation and avoid ingestion.
How is Methyl Orange packed, shipped, and exported?
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Methyl Orange is packed securely for safe transit. Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals.
How can I request a sample or quote for Methyl Orange?
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To request a sample or quote, please contact our sales team through the website or by phone.
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