Methyl Green
Methyl Green (CAS 7114-03-6) is a vital cationic dye, primarily used as a selective stain for DNA in cell biology and histology. Its chemical formula is C27H35BrClN3·ZnCl2, with a molecular weight of 653.24 g/mol. It binds specifically to AT-rich regions of native DNA, appearing yellow in acidic to neutral media and blue when bound. Methyl Green is an indispensable reagent for microscopic examination of cell nuclei.
- IUPAC
- Ethyl Green
- Synonyms
- Ethyl Green
DNA Staining
Methyl Green is employed as a marker dye for DNA in both acid and neutral conditions. It exhibits selective binding to the AT-rich regions of native DNA, enabling clear visualization of nuclear structures during microscopy.
Histology and Cytology
This dye is a valuable tool in hematology and histology for differential staining. Its ability to stain DNA allows for the differentiation of cell nuclei from cytoplasm, aiding in pathological and biological studies.
Microscopy Reagent
Utilized in various microscopy techniques, Methyl Green provides contrast and specificity for DNA, enhancing the diagnostic capabilities in research and clinical laboratories. It is particularly useful for studying chromatin structure.
| Molecular weight | 653.24 |
|---|---|
| Linear formula | C27H35BrClN3 · ZnCl2 |
| Application | Marker dye for DNA in acid and neutral media. Binds selectively to AT-rich regions of native DNA1 |
| Melting point | >300 °C(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 42590 |
|---|---|
| Absorption | λmax 423 nm (2nd)λmax 629 nm |
| Ph Range | 0.2 - 1.8, yellow to blue |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Methyl Green used for?
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Methyl Green is primarily used as a marker dye for DNA in acid and neutral media, selectively binding to AT-rich regions of native DNA. It is applied in cell biology, hematology, and histology for staining cell nuclei.
What are the CAS number and formula for Methyl Green?
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The CAS number for Methyl Green is 7114-03-6, and its linear formula is C27H35BrClN3 · ZnCl2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Methyl Green in a grade suitable for research and laboratory applications. The assay and purity are detailed on the product specification sheet. We do not represent this product as USP, BP, or EP grade unless explicitly stated.
What are the safety and handling precautions for Methyl Green?
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Methyl Green is classified with GHS07 (Warning) and may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). It is recommended to use a dust mask type N95, eyeshields, and gloves during handling. Ensure good ventilation.
How is Methyl Green shipped and exported?
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Tech Serve Solutions, as a specialist global supplier, exports Methyl Green following all international shipping regulations for chemicals. Details regarding packaging and shipping can be provided upon request.
How can I request a sample or quote for Methyl Green?
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To request a sample or a quote for Methyl Green, please contact our sales department through the website or by phone. Provide your contact details and the quantity required.
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