L-Amethopterin hydrate
L-Amethopterin hydrate (CAS: 133073-73-1, C20H22N8O5 · xH2O) is a potent inhibitor of dihydrofolate reductase, a crucial enzyme in cellular metabolism. This compound is widely utilised in antitumor research and as a tool in biological studies. Its ability to block dihydrofolate reductase makes it valuable for inhibiting this enzyme in specific experimental systems, particularly those involved in protein expression.
- IUPAC
- 4-Amino-10-methylfolic acid hydrate,L-4-Amino-N10-methylpteroylglutamic acid hydrate,L-Amethopterin hydrate,Antifolan hydrate,MTX hydrate,Methotrexate hydrate,Methylaminopterin hydrate
- Synonyms
- 4-Amino-10-methylfolic acid hydrate,L-4-Amino-N10-methylpteroylglutamic acid hydrate,L-Amethopterin hydrate,Antifolan hydrate,MTX hydrate,Methotrexate hydrate,Methylaminopterin hydrate
Antitumor Research
L-Amethopterin hydrate serves as an agent in antitumor studies due to its potent inhibition of dihydrofolate reductase. This biological activity is fundamental to its role in exploring cancer treatment strategies.
DHFR Inhibition in Biological Systems
This compound is effectively used to inhibit dihydrofolate reductase (DHFR) activity within specific experimental setups, such as DHFR-based protein expression systems. This allows researchers to study the consequences of DHFR blockade.
Antiparasitic Studies
L-Amethopterin hydrate has demonstrated efficacy in the treatment of certain malaria parasites, including pyrimethamine-resistant strains of *Plasmodium vivax*. Its mechanism involves interfering with essential metabolic pathways in the parasite.
| Molecular weight | 454.44 (anhydrous basis) |
|---|---|
| Empirical formula | C20H22N8O5 · xH2O |
| Assay | 98% |
| Solubility | H2O: insoluble |
| Optical activity | [α]25/D +17°, c = 1 in 0.05 M Na2CO3 |
| Storage temperature | −20°C |
| Application | Potent inhibitor of dihydrofolate reductase1 and agent for antitumor studies.2,3 Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites. |
| Suitability | meets USP testing specifications |
Hazard statements
- H301Toxic if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H360May damage fertility or the unborn child
Precautionary statements
- P201Obtain special instructions before use
- P301IF SWALLOWED
- P305IF IN EYES
- P308IF exposed or concerned
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 1544 6.1/PG 3 |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 61-25-36/38 |
| Safety statements (S) | 53-26-36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Gene Information | human ... DHFRP1(573971), FPGS(2356), SLC19A1(6573), TYMS(7298)mouse ... Dhfr(13361)rat ... Dhfr(24312) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Amethopterin hydrate primarily used for?
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L-Amethopterin hydrate is primarily used as a potent inhibitor of dihydrofolate reductase (DHFR) in research settings, particularly for antitumor studies and in DHFR-based protein expression systems. It is also effective against certain malaria parasites.
What is the CAS number and chemical formula for L-Amethopterin hydrate?
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The CAS number for L-Amethopterin hydrate is 133073-73-1, and its chemical formula is C20H22N8O5 · xH2O.
What grade and purity does Tech Serve Solutions supply for L-Amethopterin hydrate?
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Tech Serve Solutions supplies L-Amethopterin hydrate with an assay of 98%. We do not represent this product as meeting USP, BP, or EP testing specifications unless explicitly stated in the product data.
How should L-Amethopterin hydrate be handled safely?
+
L-Amethopterin hydrate is classified as toxic and may cause serious health effects. Handling requires stringent safety measures, including wearing appropriate personal protective equipment such as eyeshields, face shields, and a full-face particle respirator (N100 US type). Gloves and suitable respirator cartridges (N100 US type or P1/P3 EN143) are mandatory. Refer to the safety data sheet for comprehensive handling guidelines.
How is L-Amethopterin hydrate packed and shipped?
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L-Amethopterin hydrate is packed and shipped in accordance with international regulations for hazardous materials. It is transported under UN 1544, hazard class 6.1, packing group III.
How can I request a sample or quote for L-Amethopterin hydrate?
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To request a sample or a quotation for L-Amethopterin hydrate, please contact our sales department through the TSS website or by direct inquiry, providing your specific requirements.
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