Iodoacetamide
Iodoacetamide (CAS: 144-48-9) is a chemical compound with the linear formula ICH₂CONH₂ and a molecular weight of 184.96 g/mol. It functions as an effective alkylating reagent, primarily utilised for modifying cysteine residues in peptides during sequencing. Iodoacetamide is also recognised for its role as an irreversible inhibitor of various enzymes that possess cysteine at their active site, and it can inhibit ribonuclease by reacting with histidine residues.
Peptide Sequencing
Iodoacetamide serves as an alkylating reagent for the specific modification of cysteine residues within peptide chains. This application is crucial in the process of peptide sequencing, enabling the analysis of protein structures.
Enzyme Inhibition
This compound is an irreversible inhibitor of enzymes featuring cysteine at their active site. Its reactivity extends to histidine residues, which accounts for its inhibitory effect on enzymes such as ribonuclease.
Biochemical Research
As a biochemical reagent, iodoacetamide is employed in various research settings to study enzyme mechanisms and protein structure. Its defined reactivity makes it a valuable tool for biochemical investigations.
| Molecular weight | 184.96 |
|---|---|
| Linear formula | ICH2CONH2 |
| Assay | ≥99% (NMR) |
| Grade | Vetec™ reagent grade |
| Solubility | H2O: soluble0.5 M, clear, colorless |
| Storage temperature | 2-8°C |
| Impurities tested | ≤0.0005% Phosphorus (P)≤0.1% Insoluble matter |
| Melting point | 92-95 °C(lit.) |
| Application | Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site.1 It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.2 |
Hazard statements
- H301Toxic if swallowed
- H317May cause an allergic skin reaction
- H334May cause allergy or asthma symptoms if inhaled
- H413May cause long-lasting harmful effects to aquatic life
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P301IF SWALLOWED
- P342
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | T |
| Risk statements (R) | 25-42/43-53 |
| Safety statements (S) | 22-36/37-45 |
| Cation traces | Al: ≤0.0005%Ca: ≤0.0005%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%NH4+: ≤0.05%Na: ≤0.05%Pb: ≤0.001%Zn: ≤0.0005% |
| Anion traces | chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Iodoacetamide used for?
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Iodoacetamide (CAS: 144-48-9) is primarily used as an alkylating reagent for cysteine residues in peptide sequencing and as an irreversible enzyme inhibitor.
What is the CAS number and formula for Iodoacetamide?
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The CAS number for Iodoacetamide is 144-48-9, and its linear formula is ICH₂CONH₂.
What grade and purity does Tech Serve Solutions supply for Iodoacetamide?
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Tech Serve Solutions supplies Iodoacetamide in Vetec™ reagent grade with an assay of ≥99% (NMR). We do not represent this product as USP, BP, or EP grade.
How should I safely handle Iodoacetamide?
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Iodoacetamide is a dangerous substance (Danger signal word) and can cause severe skin irritation and allergic reactions (H317), and serious eye irritation (H319, though not explicitly listed in H-statements, this is implied by general chemical properties and common GHS classifications). It is toxic if swallowed (H301) and may cause allergy or asthma symptoms or breathing difficulties if inhaled (H334). It may also cause long lasting harmful effects to aquatic life (H413). Always handle with appropriate personal protective equipment, including gloves, eye protection, and respiratory protection in a well-ventilated area. Avoid dust formation and inhalation.
Is Iodoacetamide a controlled substance?
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Based on the provided data, Iodoacetamide is not indicated as a controlled or scheduled substance.
How is Iodoacetamide packed, shipped, and exported by TSS?
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Iodoacetamide is typically stored at 2-8°C. TSS is a specialist global supplier and exporter of fine chemicals, providing appropriate packaging and shipping documentation for international export in compliance with regulations.
How can I request a quote or sample for Iodoacetamide?
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To request a quote or sample for Iodoacetamide, please contact the Tech Serve Solutions sales team via our website or direct contact details.
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