Indo 1-AM
Indo 1-AM (CAS: 112926-02-0) is a cell-permeable fluorescent probe designed for monitoring intracellular calcium (Ca2+) levels. Its chemical formula is C47H51N3O22, with a molecular weight of 1009.91 g/mol. This compound exhibits a significant shift in fluorescent emission upon binding to Ca2+, making it a valuable tool in cell biology research for studying calcium signaling pathways. It is supplied by TSS with an assay of ~95% via HPLC.
- IUPAC
- 4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester
Calcium Ion Detection
Indo 1-AM serves as a cell-permeable fluorescent probe for detecting intracellular calcium ions (Ca2+). Its fluorescence emission shifts significantly upon Ca2+ binding, enabling quantitative analysis of calcium dynamics in live cells.
Cell Signaling Research
Utilised in cell biology, this probe is instrumental in investigating calcium signaling pathways, crucial for numerous cellular processes including neurotransmission, muscle contraction, and cell proliferation.
Microscopy and Flow Cytometry
Its fluorescent properties make Indo 1-AM suitable for use in fluorescence microscopy and flow cytometry applications, allowing researchers to visualise and quantify calcium changes within individual cells.
| Molecular weight | 1009.91 |
|---|---|
| Empirical formula | C47H51N3O22 |
| Assay | ~95% (HPLC) |
| Solubility | acetonitrile: soluble10 mg/mL, clear |
| Storage temperature | −20°C |
| Application | Cell permeable fluorescent probe for Ca2+; lower affinity than FURA 2; gives a large shift in fluorescent emission from 482 nm to 398 nm upon Ca2+ binding. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 330 nm; λem 450 nm (with Calcium)λex 356 nm; λem 478 nm in methanol |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Indo 1-AM used for?
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Indo 1-AM is a cell-permeable fluorescent probe used to detect and quantify intracellular calcium (Ca2+) levels, aiding research in cell signaling and various cellular processes.
What are the CAS number and formula for Indo 1-AM?
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The CAS number for Indo 1-AM is 112926-02-0, and its empirical formula is C47H51N3O22.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Indo 1-AM with a purity of approximately 95% as determined by HPLC. This product is intended for research purposes and is not represented as USP, BP, EP, or any other pharmacopoeia grade.
How should Indo 1-AM be handled safely?
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When handling Indo 1-AM, it is essential to wear appropriate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 (US) respirator filter, due to its hazardous properties.
How is Indo 1-AM packed, shipped, and exported?
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Indo 1-AM is typically stored at -20°C. TSS is an experienced global supplier and exporter of fine chemicals, adhering to all necessary regulations for secure packing and international shipment.
How can I request a sample or quote for Indo 1-AM?
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To request a sample or a quote for Indo 1-AM, please contact our sales team through the website or by calling our direct line. We aim to respond promptly to all inquiries.
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