Indo 1
Indo 1 (CAS: 96314-96-4), with the empirical formula C32H26K5N3O12 and a molecular weight of 840.05, is a highly sensitive fluorescent calcium chelator and indicator. It is essential for monitoring cytoplasmic Ca2+ transients, crucial for understanding various physiological and mechanical cellular processes. Its fluorescence properties make it a valuable tool in biochemical and cellular research for detecting dynamic changes in intracellular calcium levels.
- IUPAC
- 4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt
- Synonyms
- 4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt
Calcium Ion Monitoring
Indo 1 is widely employed as a fluorescent indicator for measuring intracellular calcium (Ca2+) concentrations. Its spectral properties allow for the ratiometric determination of Ca2+ levels, providing sensitive detection of calcium transients in cells.
Cellular Physiology Research
It facilitates the study of cellular responses to various stimuli, including receptor activation, ion channel activity, and mechanical stress, by enabling real-time observation of Ca2+ signalling pathways.
Flow Cytometry and Microscopy
Indo 1 is suitable for use in flow cytometry and fluorescence microscopy applications, allowing researchers to visualise and quantify calcium dynamics in individual cells or cellular populations.
Biochemical Assays
This compound serves as a key reagent in biochemical assays designed to investigate enzyme kinetics, protein-ligand interactions, and other biological processes where calcium plays a regulatory role.
| Molecular weight | 840.05 |
|---|---|
| Empirical formula | C32H26K5N3O12 |
| Assay | ≥90% (HPLC) |
| Grade | for fluorescence |
| Storage temperature | −20°C |
| Application | Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes. |
| Suitability | in accordance for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+λex 349 nm; λem 482 nm (100mM KCI) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Indo 1 used for?
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Indo 1 is used as a fluorescent calcium chelator and indicator for monitoring cytoplasmic Ca2+ transients in biological research, particularly in studies of cellular physiology, flow cytometry, and microscopy.
What is the CAS number and formula for Indo 1?
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The CAS number for Indo 1 is 96314-96-4, and its empirical formula is C32H26K5N3O12.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Indo 1 with an assay of ≥90% (HPLC), suitable for fluorescence applications. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling requirements for Indo 1?
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Indo 1 requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The product has a WGK Germany rating of 3.
How is Indo 1 packed, shipped, and exported?
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Indo 1 is typically stored at -20°C. As a global supplier, Tech Serve Solutions expertly packs, ships, and exports chemicals worldwide, adhering to all relevant international regulations.
How can I request a sample or quote for Indo 1?
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To request a sample or a quote for Indo 1, please contact our sales team through the website or by phone, providing your specific requirements.
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