Gram-Hucker’s stain solution (III)
Gram-Hucker’s stain solution (III) (CAS: 477-73-6), with the empirical formula C20H19ClN4 and a molecular weight of 350.84 g/mol, is a vital staining reagent. It is primarily utilised in biological and medical laboratories for cell visualisation. Its applications span cell biology and histology, enabling researchers to discern cellular structures and tissues effectively for diagnostic and research purposes.
- IUPAC
- Gram’s safranin solution,Safranin T solution
- Synonyms
- Gram’s safranin solutionSafranin T solution

Histological Staining
This solution is employed in histological procedures to stain tissue sections. It aids in differentiating cellular components, making structures visible under microscopy for detailed examination.
Cell Biology Research
In cell biology, Gram-Hucker’s stain solution (III) is used to visualise cellular morphology and intracellular components. This assists in understanding cellular processes and abnormalities.
Diagnostic Laboratory Use
It serves as a critical reagent in diagnostic laboratories for the preparation and staining of biological samples, supporting accurate pathological assessments.
| Molecular weight | 350.84 |
|---|---|
| Empirical formula | C20H19ClN4 |
| Solubility | H2O: soluble1 mg/mL |

Hazard statements
- H226Flammable liquid and vapour
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | Eyeshields, Faceshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
|---|---|
| Flash point | 46 °C / 114.8 °F |
| Transport (UN / ADR) | UN 1170C 3 / PGIII |
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | F |
| Risk statements (R) | 11 |
| Safety statements (S) | 7-16 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Gram-Hucker’s stain solution (III) used for?
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Gram-Hucker’s stain solution (III) is used for staining in histology and cell biology, aiding in the visualisation of cellular structures and tissues for research and diagnostic purposes.
What are the CAS number and formula for Gram-Hucker’s stain solution (III)?
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The CAS number is 477-73-6 and the empirical formula is C20H19ClN4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical for laboratory and research use. The specific assay and grade are available upon request, and TSS does not represent it as USP, BP, or EP grade unless explicitly stated in the product data.
How should Gram-Hucker’s stain solution (III) be handled safely?
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This solution is flammable (H226) and requires careful handling. Use appropriate personal protective equipment, including eyeshields, faceshields, and gloves. Ensure adequate ventilation and avoid open flames or sparks. Refer to the Safety Data Sheet for comprehensive guidance.
Is Gram-Hucker’s stain solution (III) suitable for export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals and reagents, including Gram-Hucker’s stain solution (III), adhering to international shipping regulations.
How can I request a sample or quote for Gram-Hucker’s stain solution (III)?
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To request a sample or obtain a quote, please contact our sales team through the website or by phone, providing the product name and CAS number (477-73-6).
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