Gly-Arg-p-nitroanilide dihydrochloride
Gly-Arg-p-nitroanilide dihydrochloride (CAS: 125455-61-0), with the molecular formula C14H21N7O4 · 2HCl and a molecular weight of 424.28 g/mol, is a valuable chromogenic peptide substrate. It is primarily employed in biochemical research for the characterization and quantification of specific enzyme activities. This compound serves as a reliable reagent in studies involving proteases, particularly those that cleave peptide bonds within biological systems.

Enzyme Activity Assays
Utilised as a chromogenic substrate to measure the activity of proteases, such as plasmin. Enzymatic cleavage releases p-nitroanilide, allowing for spectrophotometric quantification.
Biochemical Research
A key reagent in cell biology and cell signaling studies, aiding in the investigation of protein activation and degradation pathways.
Protease Characterization
Essential for identifying and characterizing novel proteases or studying the kinetics of known enzymes involved in coagulation and other biological processes.
| Molecular weight | 424.28 |
|---|---|
| Empirical formula | C14H21N7O4 · 2HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Gly-Arg-p-nitroanilide dihydrochloride used for?
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Gly-Arg-p-nitroanilide dihydrochloride is used as a chromogenic substrate in biochemical research to quantify the activity of specific proteases, such as plasmin.
What is the CAS number and formula for Gly-Arg-p-nitroanilide dihydrochloride?
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The CAS number is 125455-61-0 and the empirical formula is C14H21N7O4 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research purposes. The specific grade and purity are detailed in the product's technical specifications, and it is not represented as USP, BP, or EP grade.
What are the safety and handling recommendations for Gly-Arg-p-nitroanilide dihydrochloride?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound has a WGK Germany rating of 3, indicating it is hazardous to water. Store at -20°C.
How is Gly-Arg-p-nitroanilide dihydrochloride shipped?
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This chemical is typically shipped and exported under conditions suitable for maintaining its stability, often requiring cold chain logistics. Please contact us for detailed shipping information.
How can I request a sample or quote for Gly-Arg-p-nitroanilide dihydrochloride?
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To request a sample or quote, please contact our sales team through the website or by phone, providing the product name and CAS number (125455-61-0).
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