Fluorescamine

CAS 38183-12-9C17H10O4Chemical Synthesis

Fluorescamine (CAS: 38183-12-9, Formula: C17H10O4) is a non-fluorescent reagent that reacts with primary amines under mild conditions to yield highly fluorescent compounds. With a molecular weight of 278.26 g/mol, it is particularly useful for the sensitive fluorometric assay of amino acids, peptides, and proteins. Its ability to effectively block newly generated amino termini also makes it valuable in protein sequencing analyses.

IUPAC: 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione

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Fluorescamine (C17H10O4) — chemical structure, CAS 38183-12-9; Chemistry, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsChemical BiologyChemical SynthesisDerivatization agentsFluorescent LabelsFluorescent ProbesLabelsParticles and StainsGeneral Protein QuantitationMolecular Biology

01 /Applications

Amino Acid and Protein Analysis

Fluorescamine is employed for the sensitive fluorometric quantification of amino acids and proteins. It reacts with primary amines to form stable, fluorescent derivatives, enabling low-background detection.

Peptide Quantification

This reagent is effective in the derivatisation of peptides, facilitating their detection and quantification through fluorescence measurements. It is suitable for general protein quantitation assays.

Protein Sequencing

Fluorescamine can be used to block newly generated amino termini during protein sequencing analysis, improving the accuracy and efficiency of the process.

Enzyme Activity Assays

It serves as a valuable tool for the fluorometric assay of proteolytic enzymes, by reacting with the amino groups released during enzymatic activity.

02 /Properties

Molecular weight	278.26
Empirical formula	C17H10O4
Assay	≥98% (TLC)
Form	powder
Colour	off-white to yellow
Solubility	acetone: soluble50 mg/mL
Application	Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses.
Melting point	153-157 °C(lit.)
Suitability	suitable for fluorescence

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

38183-12-9

MDL number

MFCD00005928

PubChem substance

24894984

Beilstein registry

921143

05 /Additional specifications

Quality Level	PREMIUM
Fluorescence	λex 234 nmλex 390 nm; λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Fluorescamine used for?

Fluorescamine is used as a non-fluorescent reagent that reacts with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. It is useful for the fluorometric assay of amino acids, proteins, proteolytic enzymes, and for blocking amino termini in protein sequence analyses.

What is the CAS number and formula for Fluorescamine?

The CAS number for Fluorescamine is 38183-12-9, and its empirical formula (Hill notation) is C17H10O4.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Fluorescamine at a PREMIUM quality level, with an assay of ≥98% (TLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.

What are the safety precautions for handling Fluorescamine?

Handling Fluorescamine requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The substance is classified with WGK Germany 3, indicating a high hazard to water.

How is Fluorescamine packed and shipped?

Fluorescamine is available as an off-white to yellow powder. Tech Serve Solutions exports fine chemicals globally, adhering to all necessary shipping and handling regulations.