Fluo 3 pentaammonium salt
Fluo 3 pentaammonium salt (CAS: 339221-91-9), with the empirical formula C36H30Cl2N2O13 · 5NH3 and a molecular weight of 854.69 g/mol, is a critical fluorescent indicator for intracellular calcium (Ca2+). This cell-impermeable compound is non-fluorescent in the absence of Ca2+. Its lower binding affinity compared to some alternatives allows for precise measurement of higher peaks in calcium transients, making it valuable in cell biology research.
- IUPAC
- Glycine, N-[2-[[[[2-[bis(carboxymethyl) amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxyy]methyl]methyl]oxy]-4-methylphenyl]-N-(carboxymethyl)-pentaammonium salt
Intracellular Calcium Measurement
Fluo 3 pentaammonium salt serves as a cell-impermeable fluorescent indicator for quantifying intracellular Ca2+ levels. Its spectral properties allow for sensitive detection of calcium transients within cells.
Calcium Signaling Studies
This compound is instrumental in studying calcium signaling pathways, particularly in neuroscience and cell biology. Its ability to measure transient calcium peaks aids in understanding cellular responses and signalling dynamics.
Cellular Research Reagent
As a research tool, Fluo 3 pentaammonium salt is used to investigate various cellular processes influenced by calcium, including neurotransmitter release, muscle contraction, and apoptosis.
| Molecular weight | 854.69 |
|---|---|
| Empirical formula | C36H30Cl2N2O13 · 5NH3 |
| Assay | ≥75% (HPLC) |
| Storage temperature | −20°C |
| Application | Cell impermeable fluorescent indicator of intracellular Ca2+; non-fluorescent in the absence of Ca2+, lower binding affinity allows measurement of higher peaks of Ca2+ transients than with FURA-2. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluo 3 pentaammonium salt used for?
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Fluo 3 pentaammonium salt is used as a cell-impermeable fluorescent indicator for intracellular calcium (Ca2+), valuable in research for measuring calcium transients and studying calcium signaling in cell biology and neuroscience.
What is the CAS number and formula for Fluo 3 pentaammonium salt?
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The CAS number is 339221-91-9, and the empirical formula is C36H30Cl2N2O13 · 5NH3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Fluo 3 pentaammonium salt with an assay of ≥75% by HPLC. This product is intended for research purposes and is not represented as USP, BP, EP, or pharmaceutical grade.
What are the safety and handling precautions for Fluo 3 pentaammonium salt?
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Users should wear appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. This compound has a WGK Germany rating of 3, indicating it is hazardous.
How is Fluo 3 pentaammonium salt shipped and exported?
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Fluo 3 pentaammonium salt is typically stored at -20°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of handling international shipments in compliance with relevant regulations.
How can I request a quote or sample for Fluo 3 pentaammonium salt?
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To request a quote or a sample of Fluo 3 pentaammonium salt, please contact our sales team through the website or direct inquiry channels provided by Tech Serve Solutions.
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