Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester)
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) (CAS: 70539-42-3) is a homobifunctional, cleavable cross-linking reagent with the molecular formula C18H20N2O12. It functions by forming stable amide bonds with primary amines, typically at a pH range of 6.5-8.5, creating a 12-atom spacer. This reagent is primarily utilised in chemical biology and synthesis for protein modification and structural analysis.
- IUPAC
- Di(N-succinimidyl) ethylene glycol disuccinate,EGS,Ethylene glycol bis(succinimidyl succinate),Ethylene glycol disuccinate di(N-succinimidyl) ester
- Synonyms
- Di(N-succinimidyl) ethylene glycol disuccinateEGSEthylene glycol bis(succinimidyl succinate)Ethylene glycol disuccinate di(N-succinimidyl) ester
Protein Modification
This reagent enables the covalent modification of proteins by cross-linking primary amine groups. The stable amide linkages formed provide a 12-atom spacer arm, facilitating structural studies and functional analyses.
Chemical Biology Research
Utilised in chemical biology for investigating molecular interactions and constructing biomolecular conjugates. Its ability to link molecules via a defined spacer is crucial for assays and structural probing.
Cross-linking Applications
As a homobifunctional cross-linker, it is employed to link molecules with similar reactive groups, such as primary amines on proteins or peptides. The resulting cross-links can be cleaved under specific conditions, allowing for reversible studies.
Bioconjugation
Suitable for bioconjugation strategies requiring a cleavable linker with a defined length. It can be used to attach labels, probes, or other molecules to biological targets.
| Molecular weight | 456.36 |
|---|---|
| Empirical formula | C18H20N2O12 |
| Form | powder |
| Solubility | acetic acid: water (1:1): ≤50 mg/mLDMF: soluble |
| Storage temperature | 2-8°C |
| Application | A homobifunctional, cleavable cross-linking reagent. Typically, coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) used for?
+
It is used as a homobifunctional, cleavable cross-linking reagent in chemical biology and synthesis, particularly for protein modification and structural analysis by linking molecules containing primary amines.
What are the CAS number and molecular formula?
+
The CAS number is 70539-42-3, and the molecular formula is C18H20N2O12.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this chemical as a reagent; the specific grade and purity are available upon request. It is not supplied as USP, BP, or EP grade unless explicitly stated in the product specifications.
How should this chemical be handled and stored?
+
Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at 2-8°C.
Is this chemical a controlled substance?
+
The provided data does not indicate that this chemical is a controlled substance.
How is the product packed and shipped?
+
Products are carefully packed and exported according to international regulations for chemical shipments.
How can I request a sample or quote?
+
Please contact our sales team directly to request a sample or a quotation for this product.
▶ Related products
1-(2-Fluorobenzyl)piperazine
C11H15FN2
Chemical Synthesis
1-(2-Fluorophenyl)biguanide hydrochloride
Chemical Synthesis
1-(2-Fluorophenyl)cyclopropanecarboxylic acid
C10H9FO2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine
C10H13FN2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine monohydrochloride
C10H13FN2 · HCl
Chemical Synthesis
1-(2-Furoyl)-1H-benzotriazole
C11H7N3O2
Chemical Synthesis
1-(2-Furoyl)piperazine
C9H12N2O2
Chemical Synthesis
1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione
Chemical Synthesis
▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis