Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

CAS 67-42-5N′Chemical Synthesis

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (CAS: 67-42-5), also known as EGTA, is a chelating agent with the molecular formula [-CH2OCH2CH2N(CH2CO2H)2]2 and a molecular weight of 380.35 g/mol. It is primarily utilised for its capacity to selectively bind and sequester metal ions, notably calcium ions in the presence of magnesium ions. This specificity makes it invaluable in various analytical and biochemical applications requiring precise control over metal ion concentrations.

IUPAC: EGTA,Egtazic acid,Ethylene-bis(oxyethylenenitrilo)tetraacetic acid,Glycol ether diamine tetraacetic acid
Synonyms: EGTAEgtazic acidEthylene-bis(oxyethylenenitrilo)tetraacetic acidGlycol ether diamine tetraacetic acid

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Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (N′) — chemical structure, CAS 67-42-5; Chemistry, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsChelatorsChemical SynthesisEDTA DerivativesSynthetic Reagents More...

01 /Applications

Calcium Ion Determination

EGTA serves as a highly effective chelating agent for the quantitative determination of calcium ions. Its ability to selectively bind calcium while having a lower affinity for magnesium allows for accurate analysis in complex biological or chemical matrices.

Biochemical Research

In biochemical studies, EGTA is employed to buffer or control free calcium ion concentrations in vitro. This is critical for investigating calcium-dependent enzymatic reactions, cellular signalling pathways, and protein-ligand interactions.

Analytical Chemistry

As a versatile chelator, EGTA is used in various analytical procedures, including complexometric titrations, where it helps to mask or remove interfering metal ions, thereby improving the accuracy and reliability of results.

02 /Properties

Molecular weight	380.35
Linear formula	[-CH2OCH2CH2N(CH2CO2H)2]2
Assay	≥98.0% (T)
Loss on drying	≤1.0% loss on drying
Application	A chelating agent useful for the determination of calcium in the presence of magnesium.
Melting point	241 °C (dec.)(lit.)~250 °C (dec.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	2

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

67-42-5

MDL number

MFCD00004291

Beilstein registry

1717370

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) primarily used for?

EGTA is primarily used as a chelating agent for the determination of calcium in the presence of magnesium, and in various biochemical applications to control free calcium ion concentrations.

What are the CAS number and molecular formula for EGTA?

The CAS number for Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid is 67-42-5. Its linear formula is [-CH2OCH2CH2N(CH2CO2H)2]2.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies EGTA with an assay of ≥98.0% (T). This product is intended for laboratory and research use and is not represented as USP, BP, or EP grade.

What safety precautions should be taken when handling EGTA?

When handling EGTA, it is recommended to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if dust is generated. The compound is classified as WGK 2 (Germany).

How is EGTA packed and shipped by TSS?

Tech Serve Solutions exports and supplies fine chemicals globally. Specific packaging details will be provided upon inquiry, adhering to international shipping regulations for chemical products.

How can I request a quote or sample for EGTA?

To request a quote or sample for Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, please contact our sales team directly through our website or by phone.