DIPSO
DIPSO (CAS: 68399-80-4), with the empirical formula C7H17NO6S and a molecular weight of 243.28 g/mol, is a high-purity biological buffer. It is recognized for its zwitterionic nature and is particularly valuable in biochemical and biological research. DIPSO offers a useful pH buffering range and exhibits low UV absorbance, making it suitable for sensitive spectrophotometric applications and cell culture media.
- IUPAC
- 3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid,N,N-Bis(2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
- Synonyms
- 3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid,N,N-Bis(2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid

Biological Buffering Agent
DIPSO is employed as a zwitterionic buffer in various biological and biochemical research applications. Its buffering capacity in the physiological pH range makes it ideal for maintaining stable pH in enzyme assays, electrophoresis, and cell culture systems.
Spectrophotometric Analysis
With minimal UV absorbance at 260 nm and 280 nm, DIPSO is a preferred buffer for spectrophotometric analyses. It ensures that the buffer itself does not interfere with the measurement of nucleic acids or proteins.
Cell Culture Media
This buffer can be incorporated into cell culture media to maintain optimal pH conditions for cell growth and viability. Its low toxicity and defined buffering capacity support sensitive cell lines.
| Molecular weight | 243.28 |
|---|---|
| Empirical formula | C7H17NO6S |
| Assay | ≥98% (titration) |
| Solubility | H2O: soluble0.1 M, clear, colorless |
| pH | 4.0-5.5 (20 °C, 0.1 M in H2O) |
| Impurities tested | ≤0.0005% Phosphorus (P)≤0.1% Insoluble matter |
| Melting point | 189-192 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Cation traces | Al: ≤0.0005%Ca: ≤0.001%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%NH4+: ≤0.05%Na: ≤0.01%Pb: ≤0.001%Zn: ≤0.0005% |
| Anion traces | chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05% |
| Ignition residue | ≤0.1% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | A0.1M/260, H2O ≤0.025A0.1M/280, H2O ≤0.025 |
|---|---|
| Useful Ph Range | 7.0 - 8.2 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is DIPSO used for?
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DIPSO is used as a biological buffer in biochemical research, cell culture, electrophoresis, and spectrophotometric analyses due to its zwitterionic nature and useful pH range of 7.0 - 8.2.
What is the CAS number and formula for DIPSO?
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The CAS number for DIPSO is 68399-80-4, and its empirical formula is C7H17NO6S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies DIPSO with a purity of ≥98% (titration). This product is supplied for research and laboratory use; TSS does not represent it as USP, BP, EP, or pharmacopoeia grade.
How should DIPSO be handled safely?
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Handle DIPSO with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter, as indicated by its safety data. Consult the Safety Data Sheet (SDS) for full details.
How is DIPSO packed and shipped?
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DIPSO is carefully packed and exported by Tech Serve Solutions according to international shipping regulations for chemicals. Specific packaging details are available upon request.
How can I request a sample or quote for DIPSO?
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To request a sample or a quote for DIPSO, please contact our sales department via the website or email, providing your contact details and quantity requirements.
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