Cyanine 5 Phosphoramidite
Cyanine 5 Phosphoramidite (CAS: 351186-76-0), with the molecular formula C60H72N4O4P, is a crucial fluorescent labeling reagent. This high-purity phosphoramidite is designed for the synthesis of oligonucleotides, enabling the incorporation of the Cyanine 5 fluorophore. Its primary use lies in molecular biology and diagnostics for labeling nucleic acids, facilitating applications such as DNA sequencing, PCR, and fluorescent in situ hybridization (FISH).
- IUPAC
- 2-[5-[1,3-Dihydro-1-[3-(4-methoxytriphenylmethoxy)propyl]-3,3-dimethyl-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-[3-[N,N-diisopropylamino(2-cyanoethoxy)phosphinoxy]propyl]-3,3-dimethyl-3H-indolium
Oligonucleotide Synthesis
Cyanine 5 Phosphoramidite is a key building block for solid-phase synthesis of DNA and RNA oligonucleotides. It allows for the precise incorporation of the Cyanine 5 dye at a specific position within the nucleic acid sequence.
Fluorescent Labeling
This reagent enables the direct fluorescent labeling of nucleic acids with the bright and photostable Cyanine 5 fluorophore. This is essential for various biological assays and imaging techniques.
Diagnostic Assays
The Cyanine 5 moiety is widely used in diagnostic kits and probes due to its strong fluorescence and spectral properties, aiding in the detection and quantification of target nucleic acid sequences.
Biotechnology Research
Researchers utilize Cyanine 5-labeled probes for applications including gene expression analysis, mutation detection, and the development of novel biosensors.
| Empirical formula | C60H72N4O4P |
|---|---|
| Assay | ≥95% (31P-NMR)≥95.0% (reversed phase HPLC) |
| Colour | clear blue0.1M Acetonitrile |
| Storage temperature | −70°C |
| Product Line | Proligo Reagents |
|---|---|
| Compatibility | configured for PerkinElmerconfigured for Polygen |
| Shipped In | dry ice |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Cyanine 5 Phosphoramidite used for?
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Cyanine 5 Phosphoramidite is used for the synthesis of Cyanine 5-labeled oligonucleotides, enabling fluorescent labeling of nucleic acids for applications in molecular biology, diagnostics, and biotechnology research.
What are the CAS number and molecular formula for Cyanine 5 Phosphoramidite?
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The CAS number for Cyanine 5 Phosphoramidite is 351186-76-0, and its molecular formula is C60H72N4O4P.
What grade and purity does Tech Serve Solutions supply for Cyanine 5 Phosphoramidite?
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Tech Serve Solutions supplies Cyanine 5 Phosphoramidite with an assay of ≥95% (31P-NMR) and ≥95.0% (reversed phase HPLC). TSS does not represent this product as USP, EP, or BP grade.
What are the safety and handling considerations for Cyanine 5 Phosphoramidite?
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Cyanine 5 Phosphoramidite has a WGK Germany classification of 3, indicating it is hazardous. Handle with appropriate personal protective equipment in a well-ventilated area, avoiding contact with skin and eyes.
How is Cyanine 5 Phosphoramidite shipped and exported?
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Cyanine 5 Phosphoramidite is shipped on dry ice to maintain its required storage temperature of -70°C. TSS can facilitate global export of this chemical.
How can I request a sample or quote for Cyanine 5 Phosphoramidite?
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To request a sample or obtain a quotation for Cyanine 5 Phosphoramidite, please contact our sales department through the TSS website or by telephone.
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