Crystal Violet
Crystal Violet (CAS: 548-62-9; Formula: C25H30N3Cl; Molar Mass: 407.98 g/mol) is a triarylmethane dye known for its vibrant purple colour. It is primarily utilised as a biological stain and a pH indicator. Additionally, it finds applications in analytical chemistry and as a component in certain laboratory reagents.
- IUPAC
- Basic Violet 3,Gentian Violet,Hexamethylpararosaniline chloride,Methyl Violet 10B
- Synonyms
- Gentian VioletHexamethylpararosaniline chlorideMethyl Violet 10BBasic Violet 3

Biological Staining
Crystal Violet is widely employed in microbiology for Gram staining, differentiating bacterial species based on their cell wall composition. It is also used in histology for staining cell nuclei and other tissue components.
pH Indicator
This dye functions as a pH indicator, exhibiting a colour change within a specific pH range. Its suitability for this purpose is confirmed by passing relevant tests.
Analytical Chemistry Reagent
It serves as a reagent in various analytical procedures, contributing to the detection and quantification of specific substances. Its precise role is often application-dependent.
| Molecular weight | 407.98 |
|---|---|
| Linear formula | C25H30N3Cl |
| Assay | ≥90.0% anhydrous basis |
| Grade | JIS special grade |
| Loss on drying | ≤7.5% loss on drying |
| Suitability | passes test for sensitivity as indicator |
| Melting point | 205 °C (dec.)(lit.) |
Precautionary statements
- P273Avoid release to the environment
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
- P501Dispose of contents and container per local regulations
| Transport (UN / ADR) | UN 3077 9/PG 3 |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn,N |
| Risk statements (R) | 22-40-41-50/53 |
| Safety statements (S) | 26-36/37/39-46-60-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 42555 |
|---|---|
| Availability | available only in Japan |
| Shelf Life | (Expiry date on the label) |
| Absorption | / passes test |
| Ivd | for in vitro diagnostic use |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Crystal Violet used for?
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Crystal Violet is predominantly used as a biological stain, notably in Gram staining for bacteria, and as a pH indicator. It also has applications in analytical chemistry.
What are the CAS number and formula for Crystal Violet?
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The CAS number for Crystal Violet is 548-62-9, and its linear formula is C25H30N3Cl.
What grade and purity does Tech Serve Solutions supply for Crystal Violet?
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Tech Serve Solutions supplies Crystal Violet in JIS special grade, with an assay of ≥90.0% on an anhydrous basis. It passes a test for sensitivity as an indicator.
How should Crystal Violet be handled safely?
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Crystal Violet is harmful if swallowed and can cause serious eye damage. It is also very toxic to aquatic life with long-lasting effects. Handle with appropriate personal protective equipment (gloves, eye protection) and avoid release into the environment.
Is Crystal Violet packed, shipped, or exported by TSS?
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Crystal Violet is available only in Japan. Please contact TSS for specific export inquiries.
How can I request a sample or quote for Crystal Violet?
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To request a sample or quote for Crystal Violet, please contact the TSS sales team directly through our website or by phone.
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