Tech Serve Solutions

Crystal Violet

CAS 548-62-9C25H30N3ClBiochemicals & Reagents

Crystal Violet (CAS: 548-62-9; Formula: C25H30N3Cl; Molar Mass: 407.98 g/mol) is a triarylmethane dye known for its vibrant purple colour. It is primarily utilised as a biological stain and a pH indicator. Additionally, it finds applications in analytical chemistry and as a component in certain laboratory reagents.

IUPAC
Basic Violet 3,Gentian Violet,Hexamethylpararosaniline chloride,Methyl Violet 10B
Synonyms
Gentian VioletHexamethylpararosaniline chlorideMethyl Violet 10BBasic Violet 3
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Crystal Violet (C25H30N3Cl) — chemical structure, CAS 548-62-9; Life Science, fine chemical supplied by Tech Serve Solutions
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01 /Applications

Biological Staining

Crystal Violet is widely employed in microbiology for Gram staining, differentiating bacterial species based on their cell wall composition. It is also used in histology for staining cell nuclei and other tissue components.

pH Indicator

This dye functions as a pH indicator, exhibiting a colour change within a specific pH range. Its suitability for this purpose is confirmed by passing relevant tests.

Analytical Chemistry Reagent

It serves as a reagent in various analytical procedures, contributing to the detection and quantification of specific substances. Its precise role is often application-dependent.

02 /Properties
Molecular weight407.98
Linear formulaC25H30N3Cl
Assay≥90.0% anhydrous basis
GradeJIS special grade
Loss on drying≤7.5% loss on drying
Suitabilitypasses test for sensitivity as indicator
Melting point205 °C (dec.)(lit.)
03 /Safety & handling

Precautionary statements

  • P273Avoid release to the environment
  • P280Wear protective gloves, clothing and eye/face protection
  • P305IF IN EYES
  • P501Dispose of contents and container per local regulations
Transport (UN / ADR)UN 3077 9/PG 3
Water hazard class (WGK, DE)3
Hazard codes (EU)Xn,N
Risk statements (R)22-40-41-50/53
Safety statements (S)26-36/37/39-46-60-61

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry
CAS number
548-62-9
Beilstein registry
3580948
05 /Additional specifications
Colour Index Number42555
Availabilityavailable only in Japan
Shelf Life(Expiry date on the label)
Absorption/ passes test
Ivdfor in vitro diagnostic use
06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Crystal Violet used for?

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Crystal Violet is predominantly used as a biological stain, notably in Gram staining for bacteria, and as a pH indicator. It also has applications in analytical chemistry.

What are the CAS number and formula for Crystal Violet?

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The CAS number for Crystal Violet is 548-62-9, and its linear formula is C25H30N3Cl.

What grade and purity does Tech Serve Solutions supply for Crystal Violet?

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Tech Serve Solutions supplies Crystal Violet in JIS special grade, with an assay of ≥90.0% on an anhydrous basis. It passes a test for sensitivity as an indicator.

How should Crystal Violet be handled safely?

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Crystal Violet is harmful if swallowed and can cause serious eye damage. It is also very toxic to aquatic life with long-lasting effects. Handle with appropriate personal protective equipment (gloves, eye protection) and avoid release into the environment.

Is Crystal Violet packed, shipped, or exported by TSS?

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Crystal Violet is available only in Japan. Please contact TSS for specific export inquiries.

How can I request a sample or quote for Crystal Violet?

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To request a sample or quote for Crystal Violet, please contact the TSS sales team directly through our website or by phone.

Need Crystal Violet in a specific grade or volume?