Coelenterazine

CAS 55779-48-1C26H21N3O3Biochemicals & Reagents

Coelenterazine (CAS: 55779-48-1; Formula: C26H21N3O3; MW: 423.46) is a bioluminescent substrate utilized in various biochemical assays. Its fluorescence properties, with an excitation maximum of 400 nm and emission maximum of 514 nm, make it valuable for detecting enzymatic activity. Coelenterazine is soluble in methanol and ethanol and is typically stored at -20°C. This compound is an essential reagent for research in molecular biology and biochemistry.

IUPAC: 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one,CLZN
Synonyms: CLZN

Email us

Coelenterazine (C26H21N3O3) — chemical structure, CAS 55779-48-1; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsFluorescent LabelsFluorescent ProbesLabelsParticles and StainsOther Fluorescent Labels

01 /Applications

Bioluminescence Assays

Coelenterazine serves as a substrate in bioluminescence assays, particularly for detecting luciferase activity. Its light-emitting properties are crucial for quantifying biological processes in vitro and in vivo.

Enzyme Activity Measurement

It is widely used to measure the activity of specific enzymes, such as luciferases, by monitoring the light produced upon reaction. This is vital in drug discovery and research.

Reporter Gene Assays

Coelenterazine is employed in reporter gene assays to monitor gene expression levels. The intensity of light emitted correlates with the level of reporter gene activity.

Imaging and Detection

Its fluorescence characteristics enable its use in various imaging techniques for biological samples, aiding in the visualization and detection of cellular events.

02 /Properties

Molecular weight	423.46
Empirical formula	C26H21N3O3
Solubility	methanol and ethanol: soluble
Storage temperature	−20°C
Suitability	corresponds for UV spectrum

03 /Safety & handling

Hazard statements

H413May cause long-lasting harmful effects to aquatic life

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

55779-48-1

05 /Additional specifications

Fluorescence	λex 400 nm; λem 514 nm in 0.1 M phosphate pH 7.0

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Coelenterazine primarily used for?

Coelenterazine is primarily used as a bioluminescent substrate in biochemical assays, particularly for detecting luciferase activity and measuring enzyme activity. It also finds application in reporter gene assays and biological imaging.

What are the CAS number and molecular formula for Coelenterazine?

The CAS number for Coelenterazine is 55779-48-1, and its molecular formula is C26H21N3O3.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Coelenterazine suitable for UV spectrum analysis. The specific assay and purity are detailed in the product specifications; TSS does not represent this product as USP, BP, or EP grade.

How should Coelenterazine be handled and stored?

Coelenterazine should be handled with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 respirator filter. It must be stored at -20°C. The product has a GHS hazard statement of H413.