Bromobimane

CAS 71418-44-5C10H11BrN2O2Biochemicals & Reagents

Bromobimane (CAS: 71418-44-5, Formula: C10H11BrN2O2, MW: 271.11) is a highly fluorescent probe utilized for the specific detection and quantification of sulfhydryl groups. This chemical reagent is invaluable in biochemical research and molecular biology for labeling thiols in proteins and other biomolecules. Supplied by Tech Serve Solutions as a powder with an assay of ≥97%, Bromobimane offers a reliable method for fluorescent labeling, facilitating downstream analysis.

IUPAC: Monobromobimane

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Bromobimane (C10H11BrN2O2) — chemical structure, CAS 71418-44-5; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsCell BiologyFluorescenceFluorescent LabelsFluorescent ProbesLabelsParticles and StainsHematology and HistologyMolecular BiologyOther Fluorescent Labels

01 /Applications

Fluorescent Labeling of Sulfhydryls

Bromobimane acts as a highly specific fluorescent probe for labeling sulfhydryl (-SH) groups found in cysteine residues of proteins and other biological thiols. This reaction results in a stable, fluorescent adduct, enabling sensitive detection and quantification.

Biochemical and Cell Biology Research

It is widely used in cell biology and biochemical research to study protein modification and function, particularly when changes in thiol content are involved. Its fluorescence allows for tracking and quantification in various biological samples.

Analytical Applications

The compound's strong fluorescence makes it suitable for analytical techniques like HPLC and flow cytometry, where precise measurement of thiol-containing compounds or proteins is required.

02 /Properties

Molecular weight	271.11
Empirical formula	C10H11BrN2O2
Assay	≥97%
Form	powder
Solubility	acetonitrile: soluble20 mg/mL
Storage temperature	−20°C
Application	Fluorescent probe for thiols

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

71418-44-5

MDL number

MFCD00036951

PubChem substance

24891769

Beilstein registry

4430959

05 /Additional specifications

Absorption	extinction/396-398 nm, H2O 4600-5100

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Bromobimane used for?

Bromobimane is primarily used as a fluorescent probe for the specific detection and quantification of sulfhydryl groups in biological molecules such as proteins. It reacts with thiols to form a fluorescent adduct, enabling sensitive analysis in biochemical and cell biology research.

What are the CAS number and formula for Bromobimane?

Bromobimane has the CAS number 71418-44-5 and the empirical formula C10H11BrN2O2.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Bromobimane as a powder with an assay of ≥97%. This product is intended for research and laboratory use and is not represented as USP, BP, EP, or other pharmacopoeia grades.

How should Bromobimane be handled and stored?

Handle Bromobimane with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. It should be stored at −20°C. Note that it has a WGK Germany rating of 3, indicating a high hazard.

How is Bromobimane typically packed, shipped, and exported?

Tech Serve Solutions professionally packs, ships, and exports fine chemicals like Bromobimane, adhering to international regulations for chemical transport to ensure safe and timely delivery globally.

How can I request a sample or quote for Bromobimane?

To request a sample or a quote for Bromobimane, please contact the Tech Serve Solutions sales team through our website or by calling our office. Please specify the quantity required.