Boc-D-Phe-Ala-Nle-p-nitroanilide
Boc-D-Phe-Ala-Nle-p-nitroanilide (CAS: 75935-65-8) is a synthetic peptide derivative with the molecular formula C29H39N5O7 and a molecular weight of 569.65 g/mol. It serves as a valuable chromogenic substrate. This compound is primarily utilised in biochemical research for the assay of specific enzyme activities, particularly those involved in protease function and cellular signalling pathways. Its chemical structure facilitates the detection of enzyme-mediated cleavage.

Chromogenic Substrate for Enzyme Assays
Boc-D-Phe-Ala-Nle-p-nitroanilide is designed as a chromogenic substrate for the quantitative determination of specific enzyme activities. Upon enzymatic cleavage, it releases a coloured product, allowing for spectrophotometric measurement.
Research in Protease Activity
This compound finds application in studies focusing on proteases, particularly elastases. It aids in understanding enzyme kinetics and identifying inhibitors or modulators of protease function in biological systems.
Cell Biology and Signalling Studies
Utilised in cell biology research, it assists in investigating cellular signalling pathways where protease activity plays a crucial role. Its use contributes to elucidating complex biological mechanisms.
| Molecular weight | 569.65 |
|---|---|
| Empirical formula | C29H39N5O7 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Boc-D-Phe-Ala-Nle-p-nitroanilide used for?
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Boc-D-Phe-Ala-Nle-p-nitroanilide is primarily used as a chromogenic substrate in biochemical research for assaying enzyme activities, particularly proteases like elastase, and in studies related to cell biology and signalling.
What is the CAS number and molecular formula?
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The CAS number for Boc-D-Phe-Ala-Nle-p-nitroanilide is 75935-65-8 and its molecular formula is C29H39N5O7.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research purposes. The specific assay and purity are detailed in the product specifications. TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia standard unless explicitly stated.
How should Boc-D-Phe-Ala-Nle-p-nitroanilide be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C. This compound has a WGK Germany rating of 3, indicating a high hazard.
Is this chemical shipped internationally?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, including Boc-D-Phe-Ala-Nle-p-nitroanilide.
How can I request a sample or quote?
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Please contact our customer service team or use the online inquiry form on the Tech Serve Solutions website to request a sample or a quote for Boc-D-Phe-Ala-Nle-p-nitroanilide.
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