Bis(4-nitrophenyl) phosphate hydrate
Bis(4-nitrophenyl) phosphate hydrate (CAS: 66777-94-4) is a chemical compound with the linear formula (O2NC6H4O)2P(O)OH. Supplied by Tech Serve Solutions, this compound, with a molecular weight of 340.18 on an anhydrous basis, serves primarily as a substrate in biochemical assays, particularly for alkaline phosphatase activity. Its properties make it a valuable reagent in enzyme research and diagnostic applications.
- IUPAC
- 4-Nitrophenyl phosphate monobasic
Enzyme Substrate
Bis(4-nitrophenyl) phosphate hydrate is widely used as a chromogenic substrate for the enzyme alkaline phosphatase (ALP). Upon enzymatic hydrolysis, it releases 4-nitrophenol, which can be quantified spectrophotometrically, allowing for the measurement of ALP activity.
Biochemical Research
This compound is instrumental in biochemical research for studying enzyme kinetics and developing diagnostic assays. Its reliability as an enzyme substrate supports investigations into cellular processes and the discovery of enzyme inhibitors.
Reagent in Diagnostics
In clinical and research diagnostics, Bis(4-nitrophenyl) phosphate hydrate is employed in assays to detect and quantify alkaline phosphatase levels, which can be indicative of various physiological and pathological conditions.
| Molecular weight | 340.18 (anhydrous basis) |
|---|---|
| Linear formula | (O2NC6H4O)2P(O)OH |
| Assay | ≥97.0% (dried material, T) |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bis(4-nitrophenyl) phosphate hydrate used for?
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Bis(4-nitrophenyl) phosphate hydrate is primarily used as a chromogenic substrate for alkaline phosphatase in biochemical assays and diagnostic research.
What is the CAS number and formula for Bis(4-nitrophenyl) phosphate hydrate?
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The CAS number is 66777-94-4 and the linear formula is (O2NC6H4O)2P(O)OH.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bis(4-nitrophenyl) phosphate hydrate with an assay of ≥97.0% (dried material). TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade.
How should Bis(4-nitrophenyl) phosphate hydrate be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves, and use a type N95 (US) or P1 (EN143) respirator filter if necessary. Store at 2-8°C.
Is Bis(4-nitrophenyl) phosphate hydrate subject to specific regulations?
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The product has a WGK Germany classification of 3, indicating it is hazardous to water. Consult local regulations for specific handling and disposal requirements.
How can I request a quote or sample?
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Please contact our sales department through our website or by phone to request a quote or a sample of Bis(4-nitrophenyl) phosphate hydrate.
How is this product packaged and exported?
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Tech Serve Solutions professionally packages and exports Bis(4-nitrophenyl) phosphate hydrate, ensuring compliance with international shipping regulations for chemical reagents.
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