Bis(4-nitrophenyl) phosphate
Bis(4-nitrophenyl) phosphate (CAS 645-15-8) is a phosphorus compound with the linear formula (O₂NC₆H₄O)₂P(O)OH and a molecular weight of 340.18. It is a white to off-white solid. This compound is primarily utilised as a substrate in biochemical research, particularly for determining enzyme activity, and in studies involving catalytic mechanisms of hydrolysis. Tech Serve Solutions supplies this chemical with a minimum purity of 99%.
- IUPAC
- Di-4-nitrophenyl hydrogenphosphate
- Synonyms
- Di-4-nitrophenyl hydrogenphosphate
Enzyme Activity Assays
Bis(4-nitrophenyl) phosphate serves as a crucial substrate for assessing the activity of phosphodiesterase enzymes, specifically utilized in studies involving root phosphodiesterases from wetland plants.
Catalytic Mechanism Studies
This compound is employed to investigate the intricate mechanisms underlying the cleavage of phosphate esters, often in conjunction with various transition metal complexes as catalysts, such as oxamido-bridged dinuclear copper(II) complexes.
Organic Synthesis
As a functionalized organophosphate, Bis(4-nitrophenyl) phosphate can serve as a building block or intermediate in the synthesis of more complex organic molecules.
| Molecular weight | 340.18 |
|---|---|
| Linear formula | (O2NC6H4O)2P(O)OH |
| Assay | 99% |
| Solubility | H2O: soluble |
| Application | Bis(4-nitrophenyl) phosphate(BNPP) has been used as substrate to determine the enzyme activity of root phosphodiesterases of wetland plants1. BNPP has been used to study the mechanism of cleavage of BNPP using oxamido-bridged dinuclear copper(II) complexes as catalysts2. |
| Melting point | 172-175 °C(lit.) |
Hazard statements
- H300Fatal if swallowed
Precautionary statements
- P264Wash thoroughly after handling
- P301IF SWALLOWED
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 25 |
| Safety statements (S) | 45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bis(4-nitrophenyl) phosphate used for?
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Bis(4-nitrophenyl) phosphate is primarily used as a substrate for determining enzyme activity, particularly that of phosphodiesterases, and for studying catalytic hydrolysis mechanisms. It is also a useful building block in organic synthesis.
What is the CAS number and formula for Bis(4-nitrophenyl) phosphate?
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The CAS number for Bis(4-nitrophenyl) phosphate is 645-15-8, and its linear formula is (O₂NC₆H₄O)₂P(O)OH.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bis(4-nitrophenyl) phosphate with a minimum assay of 99%. This product is intended for research and laboratory use; TSS does not represent it as USP, BP, or EP grade.
How should Bis(4-nitrophenyl) phosphate be handled safely?
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Bis(4-nitrophenyl) phosphate is acutely toxic if swallowed (H300) and requires careful handling. Recommended personal protective equipment includes eyeshields, faceshields, gloves, and P2 respirator cartridges. In case of accidental ingestion, seek immediate medical attention (P301 + P310).
Is Bis(4-nitrophenyl) phosphate shipped internationally?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Bis(4-nitrophenyl) phosphate, and can facilitate international shipments.
How can I request a sample or quote for Bis(4-nitrophenyl) phosphate?
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To request a sample or obtain a quote for Bis(4-nitrophenyl) phosphate, please contact our sales team through the website or by phone, providing your specific requirements.
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