(+)-Biotin N-hydroxysuccinimide ester

CAS 35013-72-0C14H19N3O5SCell Biology

(+)-Biotin N-hydroxysuccinimide ester (CAS: 35013-72-0, C14H19N3O5S) is a biotinylation reagent with a molecular weight of 341.38. This activated ester readily reacts with primary amines to form stable amide bonds. It is primarily employed in biochemical research for the modification of proteins and other biomolecules, facilitating detection, purification, and immobilization in various biological applications.

IUPAC: (+)-Biotin N-succinimidyl ester,d-Biotin NHS ester,BNHS,Biotinyl-N-hydroxy-succinimide,N-Hydroxysuccinimidobiotin,N-Succinimidyl D-biotinate,NHS-Biotin
Synonyms: (+)-Biotin N-succinimidyl esterd-Biotin NHS esterBNHSBiotinyl-N-hydroxy-succinimideN-HydroxysuccinimidobiotinN-Succinimidyl D-biotinateNHS-Biotin

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(+)-Biotin N-hydroxysuccinimide ester (C14H19N3O5S) — chemical structure, CAS 35013-72-0; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Protein Biotinylation

This reagent is used to covalently attach biotin to proteins, typically through lysine residues. The resulting biotinylated proteins can then be detected or purified using avidin or streptavidin conjugates.

Nucleic Acid Labeling

It can be utilised for the labeling of nucleic acids that have been modified to incorporate primary amine groups, enabling subsequent detection or affinity purification methods.

Surface Immobilization

The activated ester allows for the covalent attachment of biotin to surfaces functionalized with amine groups, creating biotinylated surfaces for molecular capture or assay development.

Cellular Assays

Used in cell biology research to label cellular components or to study biotin-dependent biological processes.

02 /Properties

Molecular weight	341.38
Empirical formula	C14H19N3O5S
Assay	≥95% (HPLC)
Optical activity	[α]20/D +53±1°, c = 1% in DMF
Storage temperature	−20°C
Melting point	212-214 °C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

35013-72-0

Beilstein registry

4720781

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is (+)-Biotin N-hydroxysuccinimide ester used for?

(+)-Biotin N-hydroxysuccinimide ester is used as a biotinylation reagent to covalently attach biotin to molecules containing primary amines, such as proteins and nucleic acids, for detection, purification, and immobilization in biochemical and cell biology research.

What is the CAS number and formula for (+)-Biotin N-hydroxysuccinimide ester?

The CAS number is 35013-72-0 and the empirical formula (Hill notation) is C14H19N3O5S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies (+)-Biotin N-hydroxysuccinimide ester with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, or EP grade.

How should (+)-Biotin N-hydroxysuccinimide ester be handled and stored?

Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Store at -20°C.

How is (+)-Biotin N-hydroxysuccinimide ester packed, shipped, and exported?

Chemicals are carefully packed to ensure safe transit and are exported globally by Tech Serve Solutions in compliance with international shipping regulations.