Benzidine dihydrochloride
Benzidine dihydrochloride (CAS 531-85-1) is a chemical compound with the formula C12H12N2 · 2HCl and a molecular weight of 257.16 g/mol. It is recognised as an environmental genotoxin with increased cancer risks. Benzidine dihydrochloride serves as a reference material in analytical procedures for benzidine detection and is employed in studies investigating its genotoxicity mechanisms. TSS supplies this reagent for laboratory research purposes.
- IUPAC
- 4,4′-Diaminobiphenyl dihydrochloride
- Synonyms
- 4,4′-Diaminobiphenyl dihydrochloride

Analytical Reference Material
Used as a standard in analytical methods for the detection and quantification of benzidine in environmental and biological samples. Ensures accuracy and reliability in diagnostic and monitoring procedures.
Genotoxicity Research
Employed in scientific studies to investigate the mechanisms by which benzidine exerts its genotoxic effects. Aids in understanding cellular damage and potential carcinogenesis.
Histology and Staining
May be utilised in specific histological staining techniques, particularly within hematology and cell biology, where its chromogenic properties can be exploited.
| Molecular weight | 257.16 |
|---|---|
| Empirical formula | C12H12N2 · 2HCl |
| Assay | ≥99% (titration) |
| Concentration | .08-1.2 mg/mL (lot-specific value provided) |
| Application | Benzidine (4,4′-Diaminobiphenyl) is an environmental genotoxin that posses increased risks of cancer to exposed people. Benzidine may be used as a reference material in procedures that analyze for benzidine. Benzidine can be used in studies on its mechanisms of genotoxicity. |



Hazard statements
- H302Harmful if swallowed
- H350May cause cancer
- H410Very toxic to aquatic life with long-lasting effects
Precautionary statements
- P201Obtain special instructions before use
- P273Avoid release to the environment
- P308IF exposed or concerned
- P501Dispose of contents and container per local regulations
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 3077 9 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T,N |
| Risk statements (R) | 45-22-50/53 |
| Safety statements (S) | 53-45-60-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Benzidine dihydrochloride used for?
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Benzidine dihydrochloride is used as a reference material for analysing benzidine and in studies investigating its genotoxicity mechanisms. It may also find application in specific staining procedures within histology and cell biology.
What is the CAS number and formula for Benzidine dihydrochloride?
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The CAS number for Benzidine dihydrochloride is 531-85-1, and its formula is C12H12N2 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Benzidine dihydrochloride with an assay of ≥99% (titration). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeial grade.
How should Benzidine dihydrochloride be handled safely?
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Benzidine dihydrochloride is a Danger substance (H302-H350-H410), recognised as an environmental genotoxin with increased cancer risks. Handle with extreme caution, using appropriate personal protective equipment including eyeshields, face shields, gloves, and a suitable respirator. Ensure adequate ventilation and avoid exposure.
Is Benzidine dihydrochloride a controlled substance?
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The provided data does not indicate Benzidine dihydrochloride is a controlled substance under typical regulations. However, its hazardous nature requires careful handling and compliance with relevant chemical safety regulations.
How is Benzidine dihydrochloride shipped by TSS?
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Benzidine dihydrochloride is shipped internationally as UN 3077, Environmentally hazardous substance, solid, n.o.s. (Benzidine dihydrochloride), 9, PGIII.
How can I request a quote or sample for Benzidine dihydrochloride?
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To request a quote or sample for Benzidine dihydrochloride, please contact the TSS sales team via our website or direct contact details, providing the product name and CAS number.
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