7-Ethoxycoumarin-3-carbonitrile
7-Ethoxycoumarin-3-carbonitrile (CAS: 117620-77-6, C12H9NO3) is a fluorogenic substrate with a molecular weight of 215.20. It is primarily utilized for the continuous determination of cytochrome P450 mixed-function monooxygenases. This compound is valuable in enzymatic assays due to its ability to produce a fluorescent product, 3-cyano-7-hydroxycoumarin, upon reaction, enabling sensitive detection of enzyme activity.
- IUPAC
- 3-Cyano-7-ethoxycoumarin
Fluorogenic Substrate for Cytochrome P450 Assays
7-Ethoxycoumarin-3-carbonitrile serves as a sensitive fluorogenic substrate for quantifying the activity of cytochrome P450 enzymes. Its enzymatic cleavage yields fluorescent 3-cyano-7-hydroxycoumarin, allowing for continuous monitoring of monooxygenase activity.
Enzyme Activity Monitoring
This reagent is suitable for real-time measurement of enzyme reactions, particularly dealkylation processes mediated by enzymes like CYP1A. The fluorescence detection offers superior sensitivity compared to other methods.
Biochemical Research Reagent
As a specialized biochemical, it is employed in research laboratories for studying enzyme kinetics and drug metabolism pathways. Its properties facilitate precise quantitative analysis in biological samples.
| Molecular weight | 215.20 |
|---|---|
| Empirical formula | C12H9NO3 |
| Assay | ≥99.0% (HPLC) |
| Solubility | DMSO: soluble |
| Application | This reagent is a fluorogenic substrate suitable for the continuous determination of cytochrome P450 mixed-function monooxygenases. The product of the reaction is the fluorescent compound 3-cyano-7-hydroxycoumarin (Product No. C 2737). This property has been utilized to determine the activity of CYP1A by measuring the rate of dealkylation of 3-Cyano-7-ethoxycoumarin to this fluorescent product 1. Fluorescence of 3-cyano-7-hydroxycoumarin occurs at neutral pH with excitation and emission at 408 and 450 nm, respectively 2. Fluorescent reaction product detection is at least 50-fold more sensitive than that of the product of alkyl resorufin oxidation because of greater rate of turnover of 3-Cyano-7-ethoxycoumarin 3,2. The ability to continuously monitor the enzyme reaction at pH 7 is derived from the lower pKa of the 3-cyano-7- hydroxycoumarin product compared to that for 7-ethoxycoumarin 4,5. 3-Cyano-7-ethoxycoumarin is a s |
| Suitability | suitable for fluorescence |
Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 324 nm; λem 414 nm in DMSO |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-Ethoxycoumarin-3-carbonitrile used for?
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7-Ethoxycoumarin-3-carbonitrile is used as a fluorogenic substrate for the continuous determination of cytochrome P450 mixed-function monooxygenases and for monitoring enzyme activity, particularly dealkylation reactions.
What is the CAS number and molecular formula?
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The CAS number is 117620-77-6, and the molecular formula is C12H9NO3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this compound with an assay of ≥99.0% (HPLC). We do not represent this product as USP, BP, or EP grade.
How should 7-Ethoxycoumarin-3-carbonitrile be handled?
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Handle with appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves. Avoid breathing dust and ensure adequate ventilation. It is classified as harmful if swallowed, in contact with skin, or if inhaled, and causes skin and eye irritation.
How is this chemical packed and shipped?
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Tech Serve Solutions packs and ships fine chemicals globally, adhering to all necessary regulations for safe transport.
How can I request a quote or sample?
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To request a quote or sample, please contact the Tech Serve Solutions sales team via our website or direct inquiry channels.
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