6-Fluorescein Phosphoramidite
6-Fluorescein Phosphoramidite (CAS: 204697-37-0; Formula: C46H58N3O10P) is a vital reagent for oligonucleotide synthesis, enabling the incorporation of fluorescein labels. This fluorescent dye is essential for various molecular biology applications, including DNA sequencing and labelling. With a molecular weight of 843.94 g/mol, it is supplied by Tech Serve Solutions as a white to off-white solid, configured for compatibility with synthesizers like ABI 3900 and 39X.
- IUPAC
- 6-CarboxyFluorescein-Aminohexyl Amidite,6-FAM-phosphoramidite,[(3′,6′-dipivaloylfluoresceinyl)- 6-carboxamido-hexyl]- 6-O-(2-cyanoethyl)- (N,N-diisopropyl)- phosphoramidite
- Synonyms
- 6-CarboxyFluorescein-Aminohexyl Amidite6-FAM-phosphoramidite[(3′,6′-dipivaloylfluoresceinyl)-6-carboxamido-hexyl]-6-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Oligonucleotide Labelling
6-Fluorescein Phosphoramidite is a key building block for synthesising fluorescently labelled oligonucleotides. It allows for the precise incorporation of the fluorescein moiety into DNA or RNA sequences for detection and analysis.
Fluorescence-Based Assays
The incorporated fluorescein label makes molecules detectable using fluorescence spectroscopy. This is crucial for developing and performing various biochemical and molecular biology assays where signal detection is required.
DNA Sequencing
Fluorescein-labelled primers or probes can be employed in automated DNA sequencing technologies. The fluorescence allows for real-time detection of incorporated nucleotides during the sequencing process.
Gene Expression Analysis
In applications such as quantitative PCR (qPCR) or fluorescence in situ hybridization (FISH), fluorescein-labelled probes are used to detect and quantify specific nucleic acid sequences, aiding in gene expression studies.
| Molecular weight | 843.94 |
|---|---|
| Empirical formula | C46H58N3O10P |
| Assay | ≥97% (31P-NMR)≥97.0% (reversed phase HPLC)conforms (1H-NMR) |
| Colour | solid white to off-white |
| Solubility | acetonitrile: soluble0.1 M, clear, colorless to faintly yellow |
| Storage temperature | −20°C |
| Suitability | corresponds for fluorescence |
| Packaging | pkg of 1 × 0.1 g X010131-01pkg of 1 × 0.25 g X010132-01pkg of 1 × 0.25 g X010132-01 |
|---|---|
| Compatibility | configured for ABIconfigured for |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-Fluorescein Phosphoramidite used for?
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6-Fluorescein Phosphoramidite is primarily used for the synthesis of fluorescently labelled oligonucleotides, enabling applications in DNA sequencing, fluorescence-based assays, and gene expression analysis.
What are the CAS number and chemical formula?
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The CAS number for 6-Fluorescein Phosphoramidite is 204697-37-0, and its chemical formula is C46H58N3O10P.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 6-Fluorescein Phosphoramidite with an assay of ≥97% (31P-NMR) and ≥97.0% (reversed phase HPLC), conforming to 1H-NMR. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling considerations?
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According to GHS, this compound has a WGK Germany rating of 3, indicating it is hazardous to water. It should be handled with appropriate personal protective equipment in a well-ventilated area. Consult the Safety Data Sheet for full details.
How is 6-Fluorescein Phosphoramidite packaged and shipped?
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This product is available in packages of 0.1 g and 0.25 g. It is stored at -20°C and exported by Tech Serve Solutions following all relevant regulations for chemical shipments.
How can I request a quote or sample?
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To request a quote or obtain a sample of 6-Fluorescein Phosphoramidite, please contact the Tech Serve Solutions sales team through our website or by phone.
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