5β-Cholestan-3α-ol
5β-Cholestan-3α-ol (CAS 516-92-7) is a sterol lipid with the molecular formula C27H48O and a molecular weight of 388.67 g/mol. This compound, also known as epicoprostanol, serves as a valuable biochemical reagent. Its primary application is as an internal standard in the precise analytical determination of sterols in biological samples, facilitating accurate quantification via techniques such as gas chromatography (GC) and high-performance liquid chromatography (HPLC).
- IUPAC
- Epicoprostanol
- Synonyms
- Epicoprostanol

Internal Standard for Sterol Analysis
Utilised as a reference compound in GC and HPLC methods for the accurate analysis and quantification of sterols present in biological matrices such as plasma.
Biochemical Research
A key reagent in biochemical studies involving lipids and cholesterol metabolism, aiding researchers in understanding complex biological pathways.
Chromatographic Applications
Essential for method development and validation in chromatography, ensuring reliable and reproducible results in sterol profiling.
| Molecular weight | 388.67 |
|---|---|
| Empirical formula | C27H48O |
| Assay | ≥95% |
| Application | 5β-Cholestan-3α-ol was used as internal standard for analysis of sterols in plasma by GC1 and HPLC.2 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5β-Cholestan-3α-ol used for?
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5β-Cholestan-3α-ol is primarily used as an internal standard for the analysis of sterols in plasma by GC and HPLC.
What is the CAS number and formula for 5β-Cholestan-3α-ol?
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The CAS number is 516-92-7 and the empirical formula is C27H48O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5β-Cholestan-3α-ol with a minimum purity of 95%. This is a research-grade material and is not represented as USP, BP, or EP.
What are the safety and handling recommendations for 5β-Cholestan-3α-ol?
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When handling 5β-Cholestan-3α-ol, it is essential to use appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is a risk. It carries a WGK Germany rating of 3, indicating a high hazard to water.
How is 5β-Cholestan-3α-ol packed and shipped?
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Tech Serve Solutions packs and ships chemicals, including 5β-Cholestan-3α-ol, to meet international export regulations, ensuring safe and compliant delivery globally.
How can I request a sample or quote for 5β-Cholestan-3α-ol?
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To request a sample or a quotation for 5β-Cholestan-3α-ol, please contact our sales team via the website or direct email, providing your specific requirements.
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