5,6-Diaminofluorescein
5,6-Diaminofluorescein (CAS 205391-01-1) is a fluorescent indicator with a molecular weight of 362.34 g/mol. This compound serves as a valuable reagent in biochemical research, primarily utilised for its fluorescent properties in detecting specific analytes. Its application in scientific studies allows for sensitive visualisation and quantification in various biological and chemical assays.
- IUPAC
- 4,5-Diaminofluorescein,DAF-2
- Synonyms
- 4,5-Diaminofluorescein,DAF-2

Fluorescent Indicator
Utilised as a fluorescent probe for sensitive detection and imaging in biological and chemical systems. Its spectral properties enable visualisation of cellular processes and molecular interactions.
Biochemical Reagent
Employed in research laboratories for various biochemical assays and experiments. It aids in the study of enzymatic activities and cellular signalling pathways.
Nitric Oxide Detection
Functions as a probe for the detection of nitric oxide and related species in biological samples. This capability is crucial for understanding physiological and pathological processes involving NO.
| Molecular weight | 362.34 |
|---|---|
| Empirical formula | C20H14N2O5 |
| Assay | ≥95% (HPLC) |
| Storage temperature | 2-8°C |

Hazard statements
- H302Harmful if swallowed
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 505 nm; λem 518 nm in 0.1 M Tris pH 8.0 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5,6-Diaminofluorescein used for?
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5,6-Diaminofluorescein is used as a fluorescent indicator and biochemical reagent, particularly in assays for detecting nitric oxide.
What is the CAS number and molecular formula for 5,6-Diaminofluorescein?
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The CAS number is 205391-01-1 and the empirical formula is C20H14N2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5,6-Diaminofluorescein with an assay of ≥95% (HPLC). TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade.
What are the safety precautions for handling 5,6-Diaminofluorescein?
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5,6-Diaminofluorescein is classified as a Warning substance (H302 - Harmful if swallowed). Handle with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, faceshields, and gloves. Store at 2-8°C.
How is 5,6-Diaminofluorescein packed and shipped?
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5,6-Diaminofluorescein is packed in compliance with international shipping regulations for chemicals. TSS exports globally, adhering to all necessary compliance and documentation for safe and secure transit.
How can I request a sample or quote for 5,6-Diaminofluorescein?
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To request a sample or quote, please contact the Tech Serve Solutions sales team via our website or directly by email, providing your contact details and desired quantity.
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