5,6-Diaminofluorescein diacetate
5,6-Diaminofluorescein diacetate (CAS: 205391-02-2) is a cell-permeable fluorescent probe with a molecular weight of 446.41 g/mol. As a derivative of DAF-2, it is primarily utilised for the sensitive intracellular fluorimetric detection of nitric oxide (NO) and nitric oxide synthase (NOS) activity. This compound is crucial for research applications investigating cellular signalling pathways involving NO.
- IUPAC
- DAF-2 DA
- Synonyms
- C24H18N2O7DAF-2 DA
Intracellular Nitric Oxide Detection
5,6-Diaminofluorescein diacetate serves as a key reagent for the fluorimetric assay of intracellular nitric oxide production. Its cell permeability allows for direct measurement within living cells.
Nitric Oxide Synthase (NOS) Activity Monitoring
This compound is employed to monitor the activity of nitric oxide synthase enzymes, which are responsible for NO generation in various biological systems. It enables researchers to study the regulation of NO synthesis.
Cellular Signalling Research
Due to the critical role of nitric oxide in numerous physiological and pathological processes, DAF-2 DA is valuable for investigating NO-mediated cellular signalling pathways in diverse research settings.
| Molecular weight | 446.41 |
|---|---|
| Empirical formula | C24H18N2O7 |
| Assay | ≥98% (HPLC) |
| Solubility | DMSO: H2O: <2 mg/mL |
| Storage temperature | 2-8°C |
| Application | 5,6-Diaminofluorescein diacetate (DAF-2 DA), a cell permeable analog of DAF-2, is used in intracellular fluorimetric detection of nitric oxide/NOS production. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 491 nm; λem 513 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5,6-Diaminofluorescein diacetate used for?
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5,6-Diaminofluorescein diacetate (DAF-2 DA) is used for the intracellular fluorimetric detection of nitric oxide (NO) and nitric oxide synthase (NOS) production in biological research.
What is the CAS number and formula for 5,6-Diaminofluorescein diacetate?
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The CAS number is 205391-02-2, and the empirical formula (Hill notation) is C24H18N2O7.
What grade and purity does Tech Serve Solutions supply for 5,6-Diaminofluorescein diacetate?
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Tech Serve Solutions supplies 5,6-Diaminofluorescein diacetate with a purity of ≥98% (HPLC). We do not represent this product as USP, BP, or EP grade.
How should 5,6-Diaminofluorescein diacetate be handled safely?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used when handling this compound. Refer to the safety data sheet for detailed information.
How is 5,6-Diaminofluorescein diacetate typically shipped?
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This product is typically stored at 2-8°C and shipped accordingly to maintain its integrity. Tech Serve Solutions is a global supplier and exporter of fine chemicals.
How can I request a quote or sample for 5,6-Diaminofluorescein diacetate?
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Please contact our sales team via the website or direct inquiry to request a quotation or a sample of 5,6-Diaminofluorescein diacetate.
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