4-Aminophthalhydrazide
4-Aminophthalhydrazide (CAS 3682-14-2) is a chemiluminescent compound with the molecular formula C8H7N3O2 and a molecular weight of 177.16 g/mol. It is recognised for its light-emitting properties when oxidised, often in the presence of catalysts like horseradish peroxidase. This characteristic makes it a valuable reagent in various biochemical and analytical applications, particularly where sensitive detection methods are required.
- IUPAC
- 6-Amino-2,3-dihydro-1,4-phthalazinedione,Isoluminol
- Synonyms
- Isoluminol6-Amino-2,3-dihydro-1,4-phthalazinedione

Chemiluminescent Assays
Utilised as a substrate in enzyme-linked immunosorbent assays (ELISAs) and other biochemical detection systems where light emission signifies a positive reaction, enabling highly sensitive quantification.
Biochemical Research
Serves as a tool in enzymatic studies, particularly for peroxidases, due to its ability to produce light upon enzymatic oxidation, aiding in reaction monitoring and analysis.
Analytical Reagent
Employed in analytical chemistry for developing sensitive detection methods, leveraging its chemiluminescent properties for quantitative analysis in various matrices.
| Molecular weight | 177.16 |
|---|---|
| Empirical formula | C8H7N3O2 |
| Melting point | 300 °C(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Aminophthalhydrazide used for?
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4-Aminophthalhydrazide is primarily used as a chemiluminescent reagent in various biochemical and analytical assays, especially those involving peroxidases, for sensitive detection and quantification.
What is the CAS number and molecular formula of 4-Aminophthalhydrazide?
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The CAS number for 4-Aminophthalhydrazide is 3682-14-2, and its molecular formula is C8H7N3O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Aminophthalhydrazide for research and laboratory use. The specific grade and purity are detailed on the product's Certificate of Analysis. We do not represent this product as meeting USP, BP, or EP pharmacopoeial standards.
How should 4-Aminophthalhydrazide be handled safely?
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4-Aminophthalhydrazide is classified as a Warning with hazards including skin irritation (H315), eye irritation (H319), and respiratory irritation (H335). When handling, use a dust mask (N95 type), wear eyeshields and gloves, and ensure adequate ventilation. Avoid breathing dust and contact with skin and eyes.
Is 4-Aminophthalhydrazide a controlled substance?
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Based on the provided data, 4-Aminophthalhydrazide is not listed as a controlled substance.
How is 4-Aminophthalhydrazide packed and shipped?
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Tech Serve Solutions professionally packs and ships chemicals globally, adhering to all necessary safety and regulatory requirements for international transport. Specific packaging details can be provided upon request.
How can I request a sample or quote for 4-Aminophthalhydrazide?
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To request a sample or a quote for 4-Aminophthalhydrazide, please use the contact form on our website or reach out to our sales department directly with your inquiry.
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