4-Aminoantipyrine
4-Aminoantipyrine (CAS 83-07-8), with the molecular formula C11H13N3O and a molecular weight of 203.24 g/mol, is a valuable biochemical reagent. It is primarily utilised as a chromogenic agent in enzymatic assays for the quantitative determination of analytes such as glucose. Its reactivity with phenol in the presence of peroxidase is key to its diagnostic and analytical applications.
- IUPAC
- 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one,Ampyrone
- Synonyms
- Ampyrone4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one
Glucose Determination
4-Aminoantipyrine serves as a critical reagent in enzymatic methods for quantifying glucose levels. In the presence of peroxidase, it reacts with phenolic compounds and hydrogen peroxide produced during glucose oxidation to form a coloured quinoneimine dye.
Enzymatic Assays
Beyond glucose, this compound is employed in various enzymatic assays where hydrogen peroxide is generated. It acts as a chromogenic coupler, enabling spectrophotometric detection of enzyme activity or analyte concentration.
Analytical Reagent
As a versatile analytical reagent, 4-Aminoantipyrine is integral to biochemical and clinical laboratory procedures for sensitive detection and quantification of specific substances.
| Molecular weight | 203.24 |
|---|---|
| Empirical formula | C11H13N3O |
| Application | Reagent for glucose determination in the presence of phenol and peroxidase. |
| Melting point | 105-110 °C(lit.) |
Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Aminoantipyrine used for?
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4-Aminoantipyrine is primarily used as a reagent in enzymatic assays for the determination of glucose and other analytes where hydrogen peroxide is generated, employing a colorimetric method with peroxidase.
What are the CAS number and formula for 4-Aminoantipyrine?
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The CAS number for 4-Aminoantipyrine is 83-07-8, and its molecular formula is C11H13N3O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-Aminoantipyrine typically as a laboratory reagent. For specific assay details and purity, please refer to the product's Certificate of Analysis.
How should 4-Aminoantipyrine be handled safely?
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4-Aminoantipyrine is harmful if swallowed and causes skin and eye irritation, and may cause respiratory irritation. Handle with appropriate personal protective equipment, including a dust mask (N95 recommended), eyeshields, and gloves, in a well-ventilated area.
Is 4-Aminoantipyrine a controlled substance?
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Based on the provided data, 4-Aminoantipyrine is not listed as a controlled substance.
How is 4-Aminoantipyrine packed and shipped?
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4-Aminoantipyrine is carefully packed according to industry standards for chemical reagents and is available for global export by Tech Serve Solutions.
How can I request a quote or sample for 4-Aminoantipyrine?
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To request a quote or a sample of 4-Aminoantipyrine, please contact our sales team through the website or by phone, providing your specific requirements.
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