4-Amidinophenylmethanesulfonyl fluoride hydrochloride
4-Amidinophenylmethanesulfonyl fluoride hydrochloride (CAS 74938-88-8), with the molecular formula C8H9FN2O2S · HCl and a molecular weight of 252.69 g/mol, is a reagent primarily used as an enzyme inhibitor. It acts as a potent inhibitor of serine proteases and other enzymes with a serine residue in their active site. This compound is crucial for biochemical research, particularly in studying enzyme mechanisms and protein interactions.
- IUPAC
- 4-Amidinobenzylsulfonyl fluoride hydrochloride,p-APMSF
- Synonyms
- p-APMSF
Enzyme Inhibition Studies
4-Amidinophenylmethanesulfonyl fluoride hydrochloride is widely employed to investigate the role of serine proteases in biological processes. Its ability to irreversibly inhibit these enzymes allows researchers to elucidate enzyme function and identify active site residues.
Biochemical Research Tool
This compound serves as a valuable tool in cell biology and biochemistry for probing enzyme activity and signal transduction pathways. It has been instrumental in studies concerning receptor interactions, such as its use in investigating the glucagon-receptor interaction and its effect on cAMP formation.
Protein Modification Research
As an organic covalent modifier, APMSF can be used to study proteins that possess reactive serine residues. This allows for the investigation of post-translational modifications and the functional consequences of such modifications in various biological systems.
| Molecular weight | 252.69 |
|---|---|
| Empirical formula | C8H9FN2O2S · HCl |
| Solubility | H2O: soluble50 mM (Stable when aliquoted at −20°C. Half-life = 6 minutes in pH 7.0 buffer systems.) |
| Storage temperature | −20°C |
| Application | APMSF was used to study the glucagon-receptor interaction; inhibition of cAMP formation led to the conclusion that there was a previously unexpected serine residue in the active site which may operate on the receptor by a mechanism similar to serine proteases.1 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Amidinophenylmethanesulfonyl fluoride hydrochloride used for?
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4-Amidinophenylmethanesulfonyl fluoride hydrochloride (CAS 74938-88-8) is primarily used as an enzyme inhibitor in biochemical research, particularly for studying serine proteases and their active sites. It also serves as a tool in cell biology for investigating receptor interactions and protein modification.
What is the CAS number and molecular formula?
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The CAS number for 4-Amidinophenylmethanesulfonyl fluoride hydrochloride is 74938-88-8, and its molecular formula is C8H9FN2O2S · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this chemical for research purposes. The specific grade and purity are detailed in the product specifications. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling recommendations?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used when handling 4-Amidinophenylmethanesulfonyl fluoride hydrochloride. Store at −20°C.
How is this chemical shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, offering reliable shipping and export services worldwide. Specific shipping details and international regulations compliance are handled by our logistics team.
How can I request a sample or quote?
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To request a sample or a quote for 4-Amidinophenylmethanesulfonyl fluoride hydrochloride, please contact our sales department through the website or by phone, providing your specific requirements.
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