4′,5′-Dibromofluorescein
4′,5′-Dibromofluorescein (CAS: 596-03-2) is a synthetic organic compound with the molecular formula C20H10Br2O5 and a molecular weight of 490.10 g/mol. This dibrominated derivative of fluorescein functions as a vital stain and dye. It is primarily utilized in biological and histological applications for its staining properties, aiding in the visualisation of cellular structures and tissues under microscopy.
- IUPAC
- Solvent Red 72
- Synonyms
- C20H10Br2O5Solvent Red 72

Biological Staining
4′,5′-Dibromofluorescein is employed as a stain in cell biology for the visualisation of cellular components. Its fluorescent properties allow for detailed examination of biological samples.
Histological Dye
This compound serves as a dye in histology, aiding in the differentiation and identification of tissue structures. It is valuable for preparing specimens for microscopic analysis.
Research Reagent
As a chemical reagent, 4′,5′-Dibromofluorescein is used in various laboratory research settings. Its specific chemical properties make it suitable for certain analytical procedures and experimental investigations.
| Molecular weight | 490.10 |
|---|---|
| Empirical formula | C20H10Br2O5 |
| Melting point | 270-273 °C(lit.) |

Hazard statements
- H301Toxic if swallowed
Precautionary statements
- P301IF SWALLOWED
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 25 |
| Safety statements (S) | 45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45370:1 |
|---|---|
| Absorption | λmax 450 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4′,5′-Dibromofluorescein used for?
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4′,5′-Dibromofluorescein is primarily used as a stain in cell biology and as a dye in histology for visualising cellular structures and tissues.
What is the CAS number and formula for 4′,5′-Dibromofluorescein?
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The CAS number is 596-03-2, and the empirical formula is C20H10Br2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4′,5′-Dibromofluorescein, with a minimum assay of 95%. We do not represent this product as USP, BP, or EP grade.
How should 4′,5′-Dibromofluorescein be handled?
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Handle 4′,5′-Dibromofluorescein with appropriate personal protective equipment, including eyeshields, faceshields, and gloves. A P2 respirator cartridge is recommended. Avoid ingestion, as it is toxic if swallowed (H301).
Is 4′,5′-Dibromofluorescein packed and exported by TSS?
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Yes, Tech Serve Solutions professionally packs and exports 4′,5′-Dibromofluorescein globally.
How can I request a quote or sample for 4′,5′-Dibromofluorescein?
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To request a quote or sample, please contact our sales team through the website or by calling us directly.
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