3β-DOXYL-5α-cholestane, free radical

CAS 55569-61-4C31H55NO2Chemical Synthesis

3β-DOXYL-5α-cholestane, free radical (CAS: 55569-61-4) is a stable nitroxide free radical derived from cholesterol. With a molecular weight of 473.77 g/mol, this compound serves as a valuable spin probe in Electron Paramagnetic Resonance (EPR) spectroscopy. Its applications are primarily in biophysical research, enabling the study of membrane dynamics, lipid-protein interactions, and molecular order in various biological and model systems.

IUPAC: 4′,4′-Dimethylspiro(5α-cholestane-3,2′-oxazolidin)-3′-yloxy, free radical
Synonyms: 4′,4′-Dimethylspiro(5α-cholestane-3,2′-oxazolidin)-3′-yloxy, free radical

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3β-DOXYL-5α-cholestane, free radical (C31H55NO2) — chemical structure, CAS 55569-61-4; Chemistry, fine chemical supplied by Tech Serve Solutions

Chemical SynthesisDoxyl DerivativesRadical ChemistryStable RadicalsSynthetic Reagents

01 /Applications

EPR Spectroscopy Spin Probe

Utilised as a free radical spin label for Electron Paramagnetic Resonance (EPR) studies, this compound allows for detailed investigation into the dynamics and structure of biological membranes and other complex systems.

Membrane Biophysics Research

Applied to study the transversal relaxation of nitroxides in phospholipid membranes for PELDOR analysis and to investigate the comparative effects of cholesterol on bicelle model membranes.

Spin Label for Relaxation Studies

Serves as a spin probe for studying electron spin-lattice relaxation times in aqueous dispersions, employing techniques such as saturation recovery for precise measurements.

Polymer Dynamics Investigation

Employed to probe both cooperative and local modes within poly(methyl acrylate) samples using Electron Spin Resonance (ESR) spectroscopy, providing insights into polymer chain behaviour.

Liquid Crystal Dynamics

Used to study the effects of random disorder, such as that from hydrophobic aerosil, on the order and dynamics of molecular probes within nematic liquid crystals like 5CB.

02 /Properties

Molecular weight	473.77
Empirical formula	C31H55NO2
Storage temperature	2-8°C
Application	Free radical spin label used for EPR studies.• Studying transversal relaxation of nitroxides for PELDOR in phospholipid membranes1• Comparative studies of effect of cholesterol on bicelle model membranes2• Spin probe for studying electron spin-lattice relaxation times in aqueous dispersions via saturation recovery3• Probing cooperative and local modes of poly(Me acrylate) sample using ESR4• Studying random disorder effects of hydrophyobic aerosil on order and dynamics of the probe in 5CB nematic liquid crystals5
Melting point	176-178 °C(lit.)

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

55569-61-4

MDL number

MFCD00074781

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 3β-DOXYL-5α-cholestane, free radical used for?

This compound is used as a free radical spin label for Electron Paramagnetic Resonance (EPR) studies, particularly in biophysical research to investigate membrane dynamics, lipid-protein interactions, and molecular order in various systems.

What are the CAS number and molecular formula?

The CAS number is 55569-61-4 and the empirical formula is C31H55NO2.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product for research purposes. The specific grade and purity are available upon request and detailed on the Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.

What are the safety and handling precautions for this chemical?

Appropriate personal protective equipment, including eyeshields and gloves, should be worn. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Refer to the Safety Data Sheet (SDS) for comprehensive handling and hazard information. Its WGK Germany classification is 3.

How is 3β-DOXYL-5α-cholestane, free radical typically shipped or exported?

As a specialist global supplier, Tech Serve Solutions exports chemicals worldwide. This product is typically stored at 2-8°C and shipped according to international regulations for chemical transport. Please contact us for specific shipping and export details.

How can I request a quote or sample?

To request a quote or a sample of 3β-DOXYL-5α-cholestane, free radical, please use the contact form on our website or contact our sales department directly with your inquiry, referencing the CAS number 55569-61-4.