3-Cyanoumbelliferone
3-Cyanoumbelliferone (CAS: 19088-73-4), with the molecular formula C10H5NO3 and a molecular weight of 187.15 g/mol, is a fluorescent compound. It is utilized in biochemical research, particularly in studies involving cytochrome P-450 enzymes and their associated mixed-function oxidases. Its fluorescent properties make it a valuable tool for investigating enzyme kinetics and substrate specificity in biological systems.
- IUPAC
- 3-Cyano-7-hydroxycoumarin
Enzyme Kinetics Studies
3-Cyanoumbelliferone can be employed to study the kinetics of cytochrome P-450 enzymes. Its structural similarity to known substrates allows for investigations into reaction rates and enzyme mechanisms.
Cytochrome P-450 Research
This compound serves as a tool for exploring the substrate specificity of cytochrome P-450 enzymes and related mixed-function oxidases. It aids in understanding how these enzymes interact with various molecular structures.
Fluorescence Applications
With excitation at 408 nm and emission at 450 nm in methanol, 3-Cyanoumbelliferone is suitable for fluorescence-based assays and imaging techniques in biological research.
| Molecular weight | 187.15 |
|---|---|
| Empirical formula | C10H5NO3 |
| Assay | ≥98.0% (TLC) |
| Solubility | DMF: solublealcohols: soluble |
| Application | 3-Cyano-7-hydroxycoumarin is closely related to 3-cyano-7-ethoxycoumarin which is used as a fluorometric substrate and inhibitor for cytochrome P-450 enzymes and cytochrome P-450-dependent mixed function oxidases. 3-Cyano-7-hydroxycoumarin may be useful to study the kinetics and substrate specificity of cytochrome P-450s. |
| Suitability | suitable for fluorescence |
| Melting point | ≥250 °C(lit.) |
Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Gene Information | human ... MIF(4282) |
| Fluorescence | λex 408 nm; λem 450 nm in methanol |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3-Cyanoumbelliferone used for?
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3-Cyanoumbelliferone is primarily used in biochemical research to study the kinetics and substrate specificity of cytochrome P-450 enzymes and related mixed-function oxidases, leveraging its fluorescent properties.
What is the CAS number and molecular formula for 3-Cyanoumbelliferone?
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The CAS number for 3-Cyanoumbelliferone is 19088-73-4, and its molecular formula is C10H5NO3.
What grade and purity does Tech Serve Solutions supply for 3-Cyanoumbelliferone?
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Tech Serve Solutions supplies 3-Cyanoumbelliferone with a purity of ≥98.0% (TLC). This product is not specified as USP, BP, or EP grade and is intended for research purposes.
What are the safety precautions for handling 3-Cyanoumbelliferone?
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3-Cyanoumbelliferone is classified as a Warning substance. It is harmful if swallowed, in contact with skin, or if inhaled, and causes skin and eye irritation, and may cause respiratory irritation. Appropriate personal protective equipment, including a dust mask (N95), eyeshields, and gloves, should be used.
How is 3-Cyanoumbelliferone packed, shipped, and exported?
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3-Cyanoumbelliferone is carefully packed and available for global export by Tech Serve Solutions, a specialist supplier and exporter of fine chemicals.
How can I request a sample or quote for 3-Cyanoumbelliferone?
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To request a sample or a quote for 3-Cyanoumbelliferone, please contact the Tech Serve Solutions sales team through our website or designated contact channels.
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