3-(1-Acetylacetonylazo)phthalhydrazide
3-(1-Acetylacetonylazo)phthalhydrazide (CAS: 109632-03-3), with the molecular formula C13H12N4O4, is a specialised biochemical reagent. This compound is recognised for its suitability for fluorescence applications. It is primarily utilized as an enzyme substrate, particularly in studies involving urease. Tech Serve Solutions offers this high-quality chemical for research and development purposes, ensuring reliable performance in biochemical assays.
- IUPAC
- 3-(1,4-Dioxo-1,2,3,4-tetrahydrophthalazin-5-ylazo)-2,4-pentanedione

Enzyme Substrate
This compound serves as a specific substrate for enzymatic assays. Its properties are leveraged in the study and characterisation of enzyme activity, particularly for enzymes such as urease.
Fluorescence Applications
The suitability of 3-(1-Acetylacetonylazo)phthalhydrazide for fluorescence makes it valuable in biochemical research. It can be employed in assays where fluorescent detection methods are utilised for sensitivity and specificity.
Biochemical Research
As a biochemical reagent, it aids in various research fields investigating biological processes at a molecular level. Its defined chemical structure and properties support reproducible experimental outcomes.
| Molecular weight | 288.26 |
|---|---|
| Empirical formula | C13H12N4O4 |
| Assay | ≥97.0% (HPLC) |
| Suitability | suitable for fluorescence |
| Melting point | >300 °C |

Hazard statements
- H301Toxic if swallowed
Precautionary statements
- P301IF SWALLOWED
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGIII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 25 |
| Safety statements (S) | 45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3-(1-Acetylacetonylazo)phthalhydrazide used for?
+
3-(1-Acetylacetonylazo)phthalhydrazide is primarily used as an enzyme substrate, especially for urease, and is suitable for fluorescence-based applications in biochemical research.
What is the CAS number and molecular formula?
+
The CAS number for 3-(1-Acetylacetonylazo)phthalhydrazide is 109632-03-3 and its molecular formula is C13H12N4O4.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies 3-(1-Acetylacetonylazo)phthalhydrazide with an assay of ≥97.0% (HPLC). This is a high-purity grade suitable for research; TSS does not represent it as USP, BP, or EP grade.
How should 3-(1-Acetylacetonylazo)phthalhydrazide be handled?
+
This compound is classified as Danger (GHS06) and is toxic if swallowed (H301). Handle with appropriate personal protective equipment, including eyeshields, face shields, gloves, and a P2 respirator. Ensure adequate ventilation. Refer to the Safety Data Sheet for full handling instructions.
Is this product available for export?
+
Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including 3-(1-Acetylacetonylazo)phthalhydrazide, adhering to all international shipping regulations.
How can I request a quote or sample?
+
To request a quote or a sample of 3-(1-Acetylacetonylazo)phthalhydrazide, please contact our sales team via the website or direct email, specifying the product CAS number and desired quantity.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents

(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis