25-Hydroxyvitamin D3 monohydrate
25-Hydroxyvitamin D3 monohydrate (CAS: 63283-36-3), with the molecular formula C27H44O2 · H2O and a molecular weight of 418.65, is a vital metabolite of vitamin D3. This compound is crucial in biological systems for calcium and phosphate homeostasis. It finds primary application in nutritional research and cell biology studies, aiding investigations into vitamin D metabolism and its physiological effects.
- IUPAC
- 25-Hydroxycholecalciferol

Nutritional Research
Utilised in studies investigating the role of vitamin D metabolites in nutrient absorption, bone metabolism, and overall health. Its role as a key circulating form makes it invaluable for understanding dietary impacts and deficiencies.
Cell Biology
Serves as a research tool in cell culture and molecular biology experiments. It enables the study of vitamin D receptor (VDR) signalling pathways and their influence on gene expression and cellular functions.
Biochemical Assays
Employed as a reference standard or reagent in biochemical assays. It aids in the accurate quantification and analysis of vitamin D metabolites in biological samples.
| Molecular weight | 418.65 |
|---|---|
| Empirical formula | C27H44O2 · H2O |
| Assay | ≥99.0% (HPLC) |
| Storage temperature | −20°C |


Hazard statements
- H300Fatal if swallowed
- H310Fatal in contact with skin
- H330Fatal if inhaled
- H372Causes damage to organs through prolonged exposure
Precautionary statements
- P260Do not breathe dust, fume, gas, mist, vapours or spray
- P264Wash thoroughly after handling
- P280Wear protective gloves, clothing and eye/face protection
- P284Wear respiratory protection
- P301IF SWALLOWED
- P302IF ON SKIN
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T+ |
| Risk statements (R) | 24/25-26-48/25 |
| Safety statements (S) | 28-36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 25-Hydroxyvitamin D3 monohydrate used for?
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25-Hydroxyvitamin D3 monohydrate is primarily used in nutritional research and cell biology studies to investigate vitamin D metabolism and its physiological effects, including calcium and phosphate homeostasis.
What are the CAS number and molecular formula for 25-Hydroxyvitamin D3 monohydrate?
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The CAS number is 63283-36-3, and the molecular formula is C27H44O2 · H2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 25-Hydroxyvitamin D3 monohydrate with an assay of ≥99.0% (HPLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade unless explicitly stated.
How should 25-Hydroxyvitamin D3 monohydrate be handled safely?
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This compound is highly toxic and can be fatal if swallowed, inhaled, or in contact with skin. It may also cause damage to organs through prolonged or repeated exposure. Handle with extreme caution, using appropriate personal protective equipment including eyeshields, faceshields, gloves, and a full-face particle respirator (N100 US / P3 EN143).
How is 25-Hydroxyvitamin D3 monohydrate packed and exported?
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25-Hydroxyvitamin D3 monohydrate is stored at -20°C. It is packed securely for domestic and international export, adhering to all relevant shipping regulations for hazardous materials (UN 2811, 6.1, PGII).
How can I request a sample or quote for 25-Hydroxyvitamin D3 monohydrate?
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To request a sample or a quote, please contact our sales team through the website or by calling our main office. Provide the product name and CAS number (63283-36-3) for efficient processing.
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