25-Hydroxyvitamin D2
25-Hydroxyvitamin D2 (CAS: 21343-40-8, C28H44O2) is a key metabolite of Vitamin D2. This sterol derivative is crucial in cellular biology and nutrition research, playing a vital role in calcium and phosphate homeostasis. Supplied by Tech Serve Solutions, it is primarily utilised in laboratory settings for biochemical assays and metabolic studies, contributing to advancements in understanding vitamin D pathways and related physiological processes.
- IUPAC
- 25-Hydroxyergocalciferol,25-Hydroxycalciferol
- Synonyms
- 25-Hydroxyergocalciferol25-Hydroxycalciferol

Nutrition Research
Serves as a vital research chemical for studying vitamin D metabolism and its physiological effects. It is essential for understanding nutrient absorption and bone health.
Cell Biology Studies
Utilised in cell-based assays to investigate the molecular mechanisms of vitamin D action. Aids in understanding cell differentiation, proliferation, and immune responses.
Biochemical Assays
Employed as a reference standard or substrate in various biochemical assays. Used to quantify vitamin D metabolites and study enzymatic activities related to its metabolism.
| Molecular weight | 412.65 |
|---|---|
| Empirical formula | C28H44O2 |
| Assay | ≥98.0% (HPLC) |
| Storage temperature | −70°C |


Hazard statements
- H300Fatal if swallowed
- H372Causes damage to organs through prolonged exposure
Precautionary statements
- P260Do not breathe dust, fume, gas, mist, vapours or spray
- P280Wear protective gloves, clothing and eye/face protection
- P284Wear respiratory protection
- P301IF SWALLOWED
- P310Immediately call a POISON CENTER or doctor
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 2811 6.1 / PGII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T+ |
| Risk statements (R) | 24/25-26-48/25 |
| Safety statements (S) | 28-36/37-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 25-Hydroxyvitamin D2 used for?
+
25-Hydroxyvitamin D2 is used in nutrition research, cell biology studies, and biochemical assays to investigate vitamin D metabolism and its physiological roles.
What is the CAS number and chemical formula for 25-Hydroxyvitamin D2?
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The CAS number is 21343-40-8 and the chemical formula is C28H44O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 25-Hydroxyvitamin D2 with a purity of ≥98.0% (HPLC). This is a research-grade chemical and is not represented as USP, BP, or EP grade.
How should 25-Hydroxyvitamin D2 be handled and stored?
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25-Hydroxyvitamin D2 is classified as highly toxic and may be fatal if swallowed, in contact with skin, or if inhaled (H300 +H310 + H330). It also causes damage to organs through prolonged or repeated exposure (H372). Handle with extreme caution in a well-ventilated area or fume hood, using appropriate personal protective equipment including eyeshields, gloves, and a P3 respirator. Store at −70°C.
Is 25-Hydroxyvitamin D2 packed and exported by TSS?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including 25-Hydroxyvitamin D2, adhering to all relevant shipping regulations, such as UN 2811 6.1 / PGII.
How can I request a sample or quote for 25-Hydroxyvitamin D2?
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To request a sample or a quote for 25-Hydroxyvitamin D2, please contact our sales team through the TSS website or directly via email, providing your specific requirements and delivery details.
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