N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide
N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (CAS: 70967-90-7) is a synthetic peptide substrate with the empirical formula C27H38N6O9 and a molecular weight of 590.63 g/mol. This compound is primarily utilized as a sensitive chromogenic substrate for the detection and quantification of specific enzyme activities, particularly in biochemical research and diagnostics. Its reliable performance makes it a valuable tool for studying protease function and characterisation.
- IUPAC
- N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide,N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide
- Synonyms
- N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilideN-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide

Enzyme Activity Assays
This peptide is designed as a chromogenic substrate for detecting and quantifying the activity of certain proteases. The release of the p-nitroaniline chromophore upon enzymatic cleavage allows for spectrophotometric measurement of enzyme kinetics.
Biochemical Research
Used in academic and industrial research settings to investigate enzyme mechanisms, identify enzyme inhibitors, and characterise enzyme specificity, particularly for elastase and related proteases.
Cell Biology Studies
Applicable in cell biology research to understand cellular processes involving proteases, such as signal transduction, protein turnover, and extracellular matrix remodelling.
Diagnostic Reagent Development
Serves as a key component in the development of diagnostic kits for detecting enzyme levels or activities relevant to specific disease states or physiological conditions.
| Molecular weight | 590.63 |
|---|---|
| Empirical formula | C27H38N6O9 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide used for?
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N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide is primarily used as a chromogenic substrate for the detection and quantification of protease activity, particularly in biochemical research and diagnostic applications.
What is the CAS number and molecular formula for N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide?
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The CAS number is 70967-90-7 and the molecular formula is C27H38N6O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product at a PREMIUM quality level. We do not represent this product as USP, BP, or EP grade.
How should N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide be handled and stored?
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Handle with appropriate personal protective equipment including eyeshields and gloves. It is classified under WGK Germany 3. Store at −20°C.
Is this chemical controlled or scheduled?
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The provided data does not indicate that this chemical is controlled or scheduled.
How can I request a sample or quote for N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide?
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Please contact our sales team via the website or by phone to request a sample or a quotation for N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide.
How is this product packed, shipped, and exported?
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This product is carefully packed to ensure stability during transit and is available for global export. Specific packaging details can be provided upon request.
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