N-Ethylmaleimide

CAS 128-53-0C6H7NO2Biochemicals & Reagents

N-Ethylmaleimide (CAS: 128-53-0, C6H7NO2) is a crystalline compound with a molecular weight of 125.13 g/mol. It serves as a critical reagent for the covalent modification of cysteine residues within proteins. This property makes it a valuable tool in biochemical research and various industrial applications requiring targeted protein modification.

IUPAC: NEM

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N-Ethylmaleimide (C6H7NO2) — chemical structure, CAS 128-53-0; Life Science, fine chemical supplied by Tech Serve Solutions

A to CApoptosis EnzymesApoptosis InhibitorsApoptosis and Cell CycleApplication IndexBioactive Small MoleculesBiochemicals and ReagentsBromelainCalpainCalpain II

01 /Applications

Protein Modification Reagent

N-Ethylmaleimide is primarily used for the selective and covalent modification of thiol groups, specifically cysteine residues, in proteins. This reaction is fundamental in biochemical studies for protein characterisation and functional analysis.

Biochemical Research

In laboratory settings, N-Ethylmaleimide acts as an inhibitor for various enzymes, particularly those with critical cysteine residues in their active sites. It is widely employed in studies related to enzyme kinetics and protein folding pathways.

Apoptosis Studies

This compound is referenced in the context of apoptosis research, often used as an inhibitor or modulator in studies investigating cell death pathways. Its ability to react with specific protein thiols can influence cellular signaling cascades.

02 /Properties

Molecular weight	125.13
Empirical formula	C6H7NO2
Assay	≥98% (HPLC)
Form	crystalline
Boiling point	210 °C(lit.)
Solubility	95% ethanol: soluble50 mg/mL, clear to slightly hazy (colorless to faint yellow)
Storage temperature	2-8°C
Melting point	43-46 °C(lit.)
Impurities tested	≤0.001% Phosphorus (P)≤0.1% Insoluble matter
Application	Reagent for the covalent modification of cysteine residues in proteins.

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

GHS hazard pictogram: Toxic (GHS06) — Toxic

Danger

Hazard statements

H300Fatal if swallowed
H311Toxic in contact with skin
H314Causes severe skin burns and eye damage
H317May cause an allergic skin reaction

Precautionary statements

P264Wash thoroughly after handling
P280Wear protective gloves, clothing and eye/face protection
P301IF SWALLOWED
P305IF IN EYES
P310Immediately call a POISON CENTER or doctor

Protective equipment	Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges
Flash point	73 °C / 163.4 °F
Transport (UN / ADR)	UN 2928 6.1/PG 2
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T+
Risk statements (R)	21-28-34-43
Safety statements (S)	26-28-36/37/39-45
Cation traces	Al: ≤0.0005%Ca: ≤0.0005%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%Na: ≤0.005%Pb: ≤0.001%Zn: ≤0.0005%
Anion traces	chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05%
Ignition residue	≤0.1%

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

128-53-0

MDL number

MFCD00005509

PubChem substance

24278420

Beilstein registry

112448

05 /Additional specifications

Quality Level	PREMIUM
Gene Information	human ... KCNQ2(3785), KCNQ4(9132), KCNQ5(56479), SLC6A3(6531), SLC6A4(6532)

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is N-Ethylmaleimide used for?

N-Ethylmaleimide is used as a reagent for the covalent modification of cysteine residues in proteins and as an inhibitor in biochemical research, including studies on apoptosis.

What are the CAS number and formula for N-Ethylmaleimide?

The CAS number for N-Ethylmaleimide is 128-53-0, and its molecular formula is C6H7NO2.

What grade and purity does Tech Serve Solutions supply for N-Ethylmaleimide?

Tech Serve Solutions supplies N-Ethylmaleimide at a minimum purity of ≥98% (HPLC), conforming to our PREMIUM quality level. We do not represent this product as USP, BP, EP, or for pharmaceutical/nutraceutical use unless explicitly stated.

What are the safety considerations when handling N-Ethylmaleimide?

N-Ethylmaleimide is a dangerous substance classified with GHS05 and GHS06 symbols, with the signal word 'Danger'. It carries hazard statements including H300 (Fatal if swallowed), H311 (Toxic in contact with skin), H314 (Causes severe skin burns and eye damage), and H317 (May cause an allergic skin reaction). Appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection, is essential.

How is N-Ethylmaleimide packed and shipped?

N-Ethylmaleimide is packed in crystalline form and adheres to UN 2928 6.1/PG 2 shipping regulations. For export, TSS ensures compliance with all relevant international shipping and handling protocols for hazardous materials.

How can I request a quote or sample of N-Ethylmaleimide?

To request a quote or a sample of N-Ethylmaleimide, please use the contact form on our website or contact our sales department directly. Please specify the quantity required.