Dihydrorhodamine 123

CAS 109244-58-8C21H18N2O3Biochemicals & Reagents

Dihydrorhodamine 123 (CAS: 109244-58-8, Formula: C21H18N2O3) is a fluorescent probe with a molecular weight of 346.38 g/mol. This powder form compound exhibits good solubility in DMSO. It is primarily utilised in biological research as a substrate for enzyme activity detection and as a fluorescent stain for cell-based assays, aiding in the visualisation and quantification of cellular processes. Its fluorescent properties make it valuable in various imaging and flow cytometry applications.

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Dihydrorhodamine 123 (C21H18N2O3) — chemical structure, CAS 109244-58-8; Life Science, fine chemical supplied by Tech Serve Solutions

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01 /Applications

Enzyme Activity Detection

Dihydrorhodamine 123 serves as a sensitive substrate for certain enzymes, producing a fluorescent signal upon enzymatic cleavage. This allows for the detection and quantification of enzyme activity in biological samples.

Cellular Staining and Imaging

As a fluorescent probe, it can be used to stain cells, enabling visualisation of cellular structures or processes under fluorescence microscopy. Its properties are particularly useful in flow cytometry for cell analysis.

Biochemical Research

This compound is employed in biochemical investigations to study enzyme kinetics, cellular metabolism, and other biological phenomena where fluorescence-based detection is advantageous.

02 /Properties

Molecular weight	346.38
Empirical formula	C21H18N2O3
Assay	≥95%
Form	powder
Solubility	DMSO: >5 mg/mL
Storage temperature	−20°C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

109244-58-8

MDL number

MFCD00285712

PubChem substance

24893337

Beilstein registry

5989965

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Dihydrorhodamine 123 used for?

Dihydrorhodamine 123 is used as a fluorescent substrate for enzyme activity detection and as a fluorescent stain in cell biology, particularly in flow cytometry and microscopy applications.

What is the CAS number and formula for Dihydrorhodamine 123?

The CAS number for Dihydrorhodamine 123 is 109244-58-8, and its molecular formula is C21H18N2O3.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Dihydrorhodamine 123 with an assay of ≥95%. We do not represent this product as meeting USP, BP, EP, or other pharmacopoeia standards.

How should Dihydrorhodamine 123 be handled and stored?

Handle Dihydrorhodamine 123 with appropriate personal protective equipment, including eyeshields and gloves, and use a P1 (EN143) or N95 (US) respirator filter. Store at -20°C.

Is Dihydrorhodamine 123 a controlled substance?

The provided data does not indicate that Dihydrorhodamine 123 is a controlled substance.

How is Dihydrorhodamine 123 packed and shipped?

Dihydrorhodamine 123 is supplied as a powder and is packed and shipped according to standard chemical handling procedures. Tech Serve Solutions is a global supplier and exporter.