D-Val-Leu-Arg p-nitroanilide diacetate salt
D-Val-Leu-Arg p-nitroanilide diacetate salt (CAS 64816-14-4) is a synthetic tripeptide derivative with the molecular formula C23H38N8O5 · 2 C2H4O2 and a molecular weight of 626.70 g/mol. This compound serves as a chromogenic substrate in biochemical research. It is primarily used for the detection and quantification of specific enzyme activities, particularly serine proteases like thrombin, within biological samples and in enzyme kinetics studies.
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Chromogenic Substrate for Protease Assays
D-Val-Leu-Arg p-nitroanilide diacetate salt is utilised as a specific chromogenic substrate to assay the activity of certain serine proteases. Upon enzymatic cleavage, it releases p-nitroaniline, which can be quantified spectrophotometrically.
Enzyme Kinetics Research
This peptide derivative is employed in studies investigating enzyme kinetics, enabling researchers to determine kinetic parameters such as Vmax and Km for relevant proteases. Its use facilitates a deeper understanding of enzyme mechanisms and interactions.
Biochemical Research Tool
As a specialised biochemical reagent, it aids in various research applications requiring the detection or characterisation of protease activity in complex biological mixtures. Its well-defined structure supports reproducible experimental outcomes.
| Molecular weight | 626.70 |
|---|---|
| Empirical formula | C23H38N8O5 · 2 C2H4O2 |
| Assay | ≥95% (HPLC) |
| Storage temperature | −20°C |
| Composition | Peptide content, ~70% |

Hazard statements
- H361Suspected of damaging fertility or the unborn child
Precautionary statements
- P281Use personal protective equipment as required
| Protective equipment | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is D-Val-Leu-Arg p-nitroanilide diacetate salt used for?
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D-Val-Leu-Arg p-nitroanilide diacetate salt is used as a chromogenic substrate for the detection and quantification of specific protease activities, particularly serine proteases, in biochemical and enzyme kinetics research.
What are the CAS number and formula for this compound?
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The CAS number for the free base is 64816-14-4, and the empirical formula for the diacetate salt is C23H38N8O5 · 2 C2H4O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies D-Val-Leu-Arg p-nitroanilide diacetate salt with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should D-Val-Leu-Arg p-nitroanilide diacetate salt be handled safely?
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This compound is classified with GHS08 and a Warning signal word, carrying the hazard statement H361, indicating it may damage fertility or the unborn child. Appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection (type N100 or P3), is required. Handle with care, following P281 precautionary statements.
Is D-Val-Leu-Arg p-nitroanilide diacetate salt a controlled substance?
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The provided safety data does not indicate that D-Val-Leu-Arg p-nitroanilide diacetate salt is a controlled substance.
How is D-Val-Leu-Arg p-nitroanilide diacetate salt packed, shipped, and exported?
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Tech Serve Solutions professionally packs, handles, and exports fine chemicals globally, adhering to all relevant regulations for safe transport and international trade.
How can I request a sample or quote?
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To request a sample or a quote for D-Val-Leu-Arg p-nitroanilide diacetate salt, please contact our sales team through the website or by direct inquiry.
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