Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester
Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester (CAS: 89889-52-1; Formula: C26H41N5O7S; Molar Mass: 567.70 g/mol) is a specialised biotinylation reagent. It incorporates an extended 14-atom spacer arm, which can minimise steric hindrance and enhance detection sensitivity when binding to avidin or streptavidin. This compound is primarily utilised in molecular biology, protein modification, and proteomics research for labelling amine groups.
- IUPAC
- 6-(Biotinamidocaproylamido)caproic acid N-hydroxysuccinimide ester,Biotin-X-X-NHS,N-Succinimidyl N-[6-(biotinylamino)caproyl]-6-aminocaproate
- Synonyms
- 6-(Biotinamidocaproylamido)caproic acid N-hydroxysuccinimide esterBiotin-X-X-NHSN-Succinimidyl N-[6-(biotinylamino)caproyl]-6-aminocaproate
Amine Biotinylation
This reagent is ideal for introducing biotin labels onto primary amines within molecules. The reaction typically occurs efficiently in a pH range of 6.5-8.5, making it suitable for biological applications.
Protein Modification and Analysis
Its structure allows for effective biotinylation of proteins, facilitating downstream detection, purification, or structural analysis through avidin-biotin interactions. The extended spacer can improve accessibility.
Proteomics Research
In proteomics, this compound aids in tagging proteins for various analyses, including Western blotting, mass spectrometry, and protein-protein interaction studies, often increasing assay sensitivity.
Molecular Biology Tools
Used as a reagent for labelling nucleic acids or other biomolecules containing amine groups, enabling various molecular biology techniques that rely on biotin detection.
| Molecular weight | 567.70 |
|---|---|
| Empirical formula | C26H41N5O7S |
| Assay | ≥95% (TLC) |
| Form | powder |
| Solubility | DMF: soluble25 mg/mL |
| Storage temperature | −20°C |
| Application | Biotinylation reagent incorporating an extended 14-atom spacer can reduce steric hindrance in binding avidin or streptavidin to some biotinylated compounds and increase sensitivity for detection. Typically coupled to primary amine in the pH range 6.5-8.5. |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester used for?
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This compound is a biotinylation reagent used to label primary amines in molecules, particularly in protein modification, structural analysis, proteomics, and molecular biology research. Its extended spacer arm can enhance detection sensitivity.
What are the CAS number and formula for this product?
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The CAS number is 89889-52-1 and the empirical formula (Hill notation) is C26H41N5O7S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥95% (TLC). This product is intended for research purposes and TSS does not represent it as USP, BP, or EP grade.
What are the safety and handling considerations?
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This chemical has a Water Hazard Class (WGK) of 3 in Germany, indicating it is hazardous to aquatic life. Handle with appropriate personal protective equipment in a well-ventilated area. Consult the Safety Data Sheet for detailed information.
How is this chemical shipped and exported?
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Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals, capable of handling shipments and export documentation for this product worldwide.
How can I request a sample or quote?
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To request a sample or a quote for Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester, please contact our sales team through the website or by phone.
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