Agarose
Agarose (CAS 9012-36-6) is a purified polysaccharide fraction from agar, widely utilized in molecular biology and biochemistry. This white to off-white powder forms a gel matrix ideal for the separation and analysis of nucleic acids. Its primary applications include gel electrophoresis, southern blotting, and cell-based assays. TSS supplies high-quality agarose for essential laboratory research and diagnostic procedures.
Nucleic Acid Separation
Agarose is extensively used in gel electrophoresis for the preparative and analytical separation of DNA and RNA molecules based on size. Its gel-forming properties create a matrix that allows for precise resolution of nucleic acid fragments.
Southern Blotting
This compound serves as a crucial medium for southern blotting, a technique used to detect specific DNA sequences within a DNA sample. The agarose gel facilitates the transfer and hybridization process.
Single Cell Gel Electrophoresis
Agarose is employed in the single cell gel electrophoresis assay (Comet assay) to assess DNA damage in individual cells. It provides a stable matrix for immobilizing cells during the electrophoretic analysis.
Transepithelial Ionic Flux Assessment
Research applications include assessing transepithelial ionic fluxes, as demonstrated in studies involving cultured neonatal rat semicircular canal epithelium, utilising agarose to maintain tissue integrity and experimental conditions.
| Loss on drying | ≤10% loss on drying |
|---|---|
| Storage temperature | 2-8°C |
| Particle size | 45-165 μm (wet) |
| Impurities tested | DNases, none detectedRNases, none detectedphosphatases, none detectedproteases, none detected |
| Application | Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay.1 It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.2 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Cation traces | Ca: ≤500 mg/kgCd: ≤10 mg/kgCo: ≤10 mg/kgCr: ≤10 mg/kgCu: ≤10 mg/kgFe: ≤10 mg/kgK: ≤500 mg/kgMg: ≤10 mg/kgMn: ≤10 mg/kgNa: ≤5000 mg/kgNi: ≤10 mg/kgPb: ≤10 mg/kgZn: ≤10 mg/kg |
| Anion traces | chloride (Cl-): ≤3000 mg/kgsulfate (SO42-): ≤6000 mg/kg |
| Ignition residue | ≤1% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Transition Temp | gel point 34-37 °C (1.5% solution) |
|---|---|
| Matrix | 4% beaded agarose |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Agarose used for?
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Agarose is primarily used for the preparative and analytical separation of nucleic acids via gel electrophoresis. It is also employed in single cell gel electrophoresis assays and southern blotting, and for assessing transepithelial ionic fluxes.
What is the CAS number and formula for Agarose?
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The CAS number for Agarose is 9012-36-6. The chemical formula is not specified in the product data.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Agarose with DNases, RNases, phosphatases, and proteases not detected. It has a loss on drying of ≤10%, and trace impurities are specified. TSS does not represent this product as USP, BP, or EP grade.
How should Agarose be stored and handled?
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Agarose should be stored at 2-8°C. Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a P1 or N95 respirator filter, due to potential dust inhalation. It is classified under WGK Germany 3.
Is Agarose a controlled substance?
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The provided data does not indicate that Agarose (CAS 9012-36-6) is a controlled substance.
How is Agarose packed and exported by TSS?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Agarose. Specific packing details for export can be provided upon request.
How can I request a sample or quote for Agarose?
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To request a sample or obtain a quote for Agarose, please contact the Tech Serve Solutions sales team through the website or designated contact channels.
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