4-Methylumbelliferyl β-D-lactopyranoside

CAS 84325-23-5C22H28O13Biochemicals & Reagents

4-Methylumbelliferyl β-D-lactopyranoside (CAS 84325-23-5), with the empirical formula C22H28O13 and a molecular weight of 500.45 g/mol, is a fluorogenic substrate. It is primarily employed in biochemical research for the detection and quantification of enzyme activity, particularly those that hydrolyse lactose. Its fluorescence properties upon enzymatic cleavage make it valuable for various analytical applications in life sciences.

IUPAC: 4-Methylumbelliferyl β-D-lactoside
Synonyms: 4-Methylumbelliferyl beta-D-lactoside4-Methylumbelliferyl beta-D-galactopyranoside-1-O-beta-D-lactopyranoside

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4-Methylumbelliferyl β-D-lactopyranoside (C22H28O13) — chemical structure, CAS 84325-23-5; Life Science, fine chemical supplied by Tech Serve Solutions

ActivityBiochemicals and ReagentsEnzyme SubstratesEnzymesInhibitorsand SubstratesFluorescent ProbesLabelsParticles and StainsFluorogenic

01 /Applications

Enzyme Activity Assays

This compound serves as a sensitive fluorogenic substrate for detecting and quantifying the activity of enzymes, such as beta-galactosidase, that cleave glycosidic bonds. The release of 4-methylumbelliferone upon hydrolysis results in fluorescence, enabling precise measurements.

Biochemical Research

It is utilised in various biochemical and glycobiology studies to investigate enzymatic pathways, enzyme kinetics, and substrate specificity. Its fluorogenic nature facilitates real-time monitoring of enzymatic reactions in vitro.

Fluorescence Microscopy and Spectroscopy

The compound's ability to emit fluorescence (λex 365 nm; λem 445 nm after degradation) makes it suitable for applications in fluorescence microscopy and spectroscopy, aiding in the visualisation and analysis of biological samples.

02 /Properties

Molecular weight	500.45
Empirical formula	C22H28O13
Storage temperature	−20°C

03 /Identifiers & registry

CAS number

84325-23-5

MDL number

MFCD00083406

PubChem substance

24896730

Beilstein registry

8094402

04 /Additional specifications

Fluorescence	λex 365 nm; λem 445 nm (after enzymatic degradation)

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 4-Methylumbelliferyl β-D-lactopyranoside used for?

4-Methylumbelliferyl β-D-lactopyranoside is used as a fluorogenic substrate in biochemical research for detecting and quantifying enzyme activity, particularly of enzymes that cleave lactose, and in fluorescence microscopy and spectroscopy.

What are the CAS number and formula for 4-Methylumbelliferyl β-D-lactopyranoside?

The CAS number is 84325-23-5, and the empirical formula is C22H28O13.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product for research and laboratory use. The specific assay and purity are detailed in the product's specification sheet. TSS does not represent this product as USP, BP, EP, or pharmaceutical grade.

What are the safety and handling precautions for this chemical?

This chemical has a WGK (Wassergefährdungsklasse) rating of 3, indicating it is hazardous to water. Appropriate personal protective equipment should be used, and it should be handled in a well-ventilated area, avoiding release into the environment.

How is this product packed, shipped, and exported?

Tech Serve Solutions exports fine chemicals globally. Product packaging adheres to international shipping regulations to ensure safe transport and handling.