3-Acetylumbelliferyl β-D-glucopyranoside
3-Acetylumbelliferyl β-D-glucopyranoside (CAS: 20943-16-2) is a fluorogenic enzyme substrate with the molecular formula C17H18O9 and a molecular weight of 366.32 g/mol. This biochemical reagent is widely used in research for the detection and quantification of beta-glucosidase activity. Its fluorescence upon enzymatic cleavage makes it invaluable in various biochemical assays and diagnostic applications, contributing to advancements in fields like glycobiology and enzyme kinetics.
- IUPAC
- 3-Acetyl-7-coumarinyl-β-D-glucopyranoside,3-Acetyl-7-(β-D-glucopyranosyloxy)coumarin
- Synonyms
- 3-Acetyl-7-(β-D-glucopyranosyloxy)coumarin3-Acetyl-7-coumarinyl-β-D-glucopyranoside

Enzyme Activity Assays
Utilised as a fluorogenic substrate to detect and measure the activity of beta-glucosidase enzymes. Its cleavage by the enzyme releases a fluorescent product, enabling sensitive quantification in various biological samples.
Glycobiology Research
A valuable tool in glycobiology for studying glycosidic bond hydrolysis and the function of enzymes involved in carbohydrate metabolism. It aids in understanding complex biological processes related to glycosylation.
Biochemical Research
Employed in general biochemical research to investigate enzyme kinetics and reaction mechanisms involving beta-glucosidases. It serves as a reliable substrate for screening enzyme inhibitors and activators.
Diagnostic Applications
Can be applied in the development of diagnostic kits and assays for detecting beta-glucosidase presence or activity in clinical or environmental samples, leveraging its fluorescent readout.
| Molecular weight | 366.32 |
|---|---|
| Empirical formula | C17H18O9 |
| Assay | ≥95% |
| Storage temperature | 2-8°C |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3-Acetylumbelliferyl β-D-glucopyranoside used for?
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3-Acetylumbelliferyl β-D-glucopyranoside is primarily used as a fluorogenic substrate for the assay of beta-glucosidase activity in biochemical research, glycobiology, and potential diagnostic applications.
What are the CAS number and molecular formula for this compound?
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The CAS number is 20943-16-2, and the molecular formula is C17H18O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3-Acetylumbelliferyl β-D-glucopyranoside with an assay of ≥95%. We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should 3-Acetylumbelliferyl β-D-glucopyranoside be handled and stored?
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Handle with appropriate personal protective equipment, including a dust mask (N95 type), eyeshields, and gloves. Store at 2-8°C. This compound is classified with a Warning signal word and may cause skin irritation (H315), eye irritation (H319), and respiratory irritation (H335).
Is 3-Acetylumbelliferyl β-D-glucopyranoside a controlled substance?
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The provided data does not indicate that 3-Acetylumbelliferyl β-D-glucopyranoside is a controlled substance.
How is this product shipped or exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of shipping and exporting 3-Acetylumbelliferyl β-D-glucopyranoside worldwide, adhering to all necessary regulations.
How can I request a sample or quote?
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To request a sample or a quote for 3-Acetylumbelliferyl β-D-glucopyranoside, please contact our sales department through the website or by phone.
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