2′-Deoxyuridine 5′-triphosphate sodium salt solution
2′-Deoxyuridine 5′-triphosphate sodium salt solution (CAS: 94736-09-1) is a vital reagent in molecular biology, with the empirical formula C9H15N2O14P3 · xNa+ and a molecular weight of 468.14 (free acid basis). This dUTP solution is essential for PCR applications, particularly for preventing carryover contamination. It functions by substituting dTTP in PCR reactions, enabling subsequent elimination of amplified products by uracil DNA glycosylase.
- IUPAC
- dUTP sodium salt
PCR Carryover Contamination Prevention
This solution is specifically formulated for use in PCR to prevent carryover contamination between experiments. By incorporating dUTP instead of dTTP, amplified DNA can be specifically degraded by uracil DNA glycosylase, ensuring clean subsequent reactions.
Molecular Biology Research
As a deoxynucleotide triphosphate (dNTP), 2′-Deoxyuridine 5′-triphosphate sodium salt is a fundamental building block for DNA synthesis and manipulation in various molecular biology techniques. Its role in preventing PCR contamination makes it particularly valuable in sensitive genomic research.
DNA Amplification Strategies
Incorporating dUTP into PCR reactions, facilitated by this reagent, is a well-established strategy for controlling amplification artifacts. This allows researchers to maintain the integrity of their experimental results, especially when working with low template concentrations or repetitive experiments.
| Molecular weight | 468.14 (free acid basis) |
|---|---|
| Empirical formula | C9H15N2O14P3 · xNa+ |
| Assay | ≥99% |
| Concentration | 100 mM in H2O |
| Storage temperature | −20°C |
| Application | Used in conjunction with uracil DNA glycosylase to prevent carryover contamination in PCR. The dUTP is substituted for dTTP in the PCR reaction. |
| Foreign Activity | DNase, RNase, none detected |
|---|---|
| Shipped In | dry ice |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2′-Deoxyuridine 5′-triphosphate sodium salt solution used for?
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This solution is primarily used in PCR applications to prevent carryover contamination by substituting dTTP, allowing for subsequent degradation of amplified products by uracil DNA glycosylase.
What is the CAS number and formula for this product?
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The CAS number is 94736-09-1, and the empirical formula is C9H15N2O14P3 · xNa+.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥99%. It is suitable for research applications; TSS does not represent it as USP, BP, or EP grade.
How should this product be handled and stored safely?
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This product has a WGK Germany rating of 3, indicating it is hazardous. It should be stored at -20°C and handled with appropriate personal protective equipment. Consult the Safety Data Sheet for detailed handling instructions.
How is this product shipped?
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This solution is shipped in dry ice to maintain its stability during transit.
How can I request a quote or sample?
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Please contact our sales team through the website to request a quote or a sample of 2′-Deoxyuridine 5′-triphosphate sodium salt solution.
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