Tricine

CAS 5704-04-1(HOCH2)3CNHCH2CO2HBiochemicals & Reagents

Tricine (CAS 5704-04-1) is a zwitterionic buffering agent with a pKa of 8.1, suitable for use in the pH range of 7.4 to 8.8. This N-tris(hydroxymethyl)methyl glycine derivative is a high-purity, synthetic compound supplied as a fine powder. It is primarily employed as a buffer component in biochemical applications, particularly for the separation of low molecular weight peptides. Its low UV absorbance characteristics make it valuable in spectroscopic analyses.

IUPAC: N-[Tris(hydroxymethyl)methyl]glycine
Synonyms: N-[Tris(hydroxymethyl)methyl]glycineTris(hydroxymethyl)methylglycine2-hydroxy-N-[2-hydroxy-1-(hydroxymethyl)ethyl]-N-(2-hydroxy-1-methylethyl)ethanamine2-hydroxy-N,N-bis(2-hydroxy-1-methylethyl)ethylamineTris glycinamide

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Tricine ((HOCH2)3CNHCH2CO2H) — chemical structure, CAS 5704-04-1; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsBiological BuffersGood’s BuffersPharmaGrade Buffers

01 /Applications

Biochemical Buffering

Tricine serves as an effective buffer in various biochemical research and analytical applications. Its zwitterionic nature and pKa value make it suitable for maintaining stable pH conditions in biological systems, particularly in the physiologically relevant range.

Peptide Separation

This compound is utilized as a buffer component in electrophoresis and chromatography for the separation of low molecular weight peptides. Its buffering capacity aids in achieving reproducible separation results.

Electrophoresis Reagent

In protein and peptide analysis, Tricine is often used in buffer systems for gel electrophoresis, such as SDS-PAGE, contributing to the resolution and migration characteristics of biomolecules.

02 /Properties

Molecular weight	179.17
Linear formula	(HOCH2)3CNHCH2CO2H
Assay	≥99% (titration)
Form	powder
Solubility	H2O: soluble1 M, clear, colorless
pH	4.0-6.0 (1 M in H2O)
Impurities tested	≤0.005% Phosphorus (P)≤0.1% Insoluble matter
Application	Buffer component for separation of low molecular weight peptides.
Biological source	from synthetic

03 /Safety & handling

Water hazard class (WGK, DE)	3
Cation traces	Al: ≤0.0005%Ca: ≤0.0005%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%NH4+: ≤0.05%Na: ≤0.005%Pb: ≤0.001%Zn: ≤0.0005%
Anion traces	chloride (Cl-): ≤0.1%sulfate (SO42-): ≤0.05%
Ignition residue	≤0.1%

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

5704-04-1

Beilstein registry

1937804

05 /Additional specifications

Quality Level	ELITE
Product Line	PharmaGrade
Pka 25 &#X00b0;C	8.1
Foreign Activity	Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
Absorption	A1M/260, H2O ≤0.1A1M/280, H2O ≤0.05
Useful Ph Range	7.4 - 8.8

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Tricine used for?

Tricine is used as a buffer component for the separation of low molecular weight peptides, and generally as a biological buffer in the pH range of 7.4 to 8.8. It is also employed in electrophoresis and other biochemical applications.

What is the CAS number and formula for Tricine?

The CAS number for Tricine is 5704-04-1. Its linear formula is (HOCH2)3CNHCH2CO2H.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Tricine at ELITE quality level with an assay of ≥99% by titration. It is designated as a PharmaGrade buffer. TSS does not represent this product as USP, BP, or EP grade.

How should Tricine be handled safely?

Tricine is classified under WGK Germany 3, indicating it is hazardous to water. Handle with appropriate personal protective equipment to avoid contact and inhalation. Refer to the Safety Data Sheet for detailed handling instructions.

How is Tricine packed and exported?

Tricine is supplied as a powder and is carefully packed to maintain its integrity during domestic and international shipment. Tech Serve Solutions is a global supplier and exporter of fine chemicals.